PMID: 9546665Apr 18, 1998Paper

Localization of carbohydrate chains of pig sperm ligand in the glycoprotein ZPB of egg zona pellucida

European Journal of Biochemistry
K KudoM Nakano

Abstract

The three glycoproteins of pig zona pellucida (ZPA, ZPB and ZPC) can be separated into ZPA and a mixture of ZPB/ZPC by gel-filtration HPLC. We have shown previously that the neutral complex-type N-linked carbohydrate chains obtained from ZPB/ZPC possess sperm-binding activity. Intact ZPB and ZPC cannot be separated from each other unless acidic N-acetyllactosamine regions of their carbohydrate chains are removed by endo-beta-galactosidase digestion. The endo-beta-galactosidase-digested ZPB retains the sperm-binding activity. Recently, we have reported that N-linked carbohydrate chains of N-terminal fragment (residues 137-247) obtained from endo-beta-galactosidase-digested ZPB are involved mainly in sperm binding [Yonezawa, N., Mitsui, S., Kudo, K. & Nakano, M. (1997) Eur. J. Biochem. 248, 86-92]. In this study, we separated the intact neutral N-linked chains from the ZPB/ZPC mixture into diantennary chains and triantennary and tetraantennary chains by affinity chromatography on Concanavalia ensiformis agglutinin. An in vitro competition assay revealed that triantennary and tetraantennary chains possess a sperm-binding activity stronger than that of diantennary chains. Three glycopeptides, having one Asn residue to which the car...Continue Reading

Citations

Jul 25, 2003·Biochemical and Biophysical Research Communications·Naoto Yonezawa, Minoru Nakano
Mar 9, 2000·Journal of Reproductive Immunology·M R Barber, R A Fayrer-Hosken
Jun 14, 2000·Animal Reproduction Science·E Töpfer-PetersenM Ekhlasi-Hundrieser
Oct 20, 2011·Zygote : the Biology of Gametes and Early Embryos·Naoto YonezawaMinoru Nakano
Aug 30, 2002·European Journal of Biochemistry·Keiichi IkedaMinoru Nakano
Mar 26, 1999·European Journal of Biochemistry·N YonezawaM Nakano
May 19, 2001·Glycobiology·K J Mengerink, V D Vacquier
Nov 19, 2008·Reproductive Biology and Endocrinology : RB&E·Sylvie MugnierGhylène Goudet
Jun 22, 2014·Biochemical and Biophysical Research Communications·Gary F Clark
Jan 28, 2015·Theriogenology·I StetsonM J Izquierdo-Rico
Mar 28, 2006·Current Topics in Developmental Biology·Julian L Wong, Gary M Wessel
Sep 25, 1999·The Journal of Experimental Zoology·E Töpfer-Petersen
Mar 7, 2020·Cell and Tissue Research·Michal ZigoPeter Sutovsky
Jul 29, 2010·Systems Biology in Reproductive Medicine·Gary F Clark
Apr 30, 1999·Physiological Reviews·A DarszonF Espinosa
Feb 5, 2021·Frontiers in Cell and Developmental Biology·Mª José Izquierdo-RicoManuel Avilés

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