Localization of caveolin-3 in the sinus endothelial cells of the rat spleen

Cell and Tissue Research
Kiyoko Uehara, Masayuki Miyoshi

Abstract

The localization of caveolins in the sinus endothelial cells of the rat spleen has been demonstrated by confocal laser scanning and electron microscopy. Caveolin-3, a muscle-specific caveolin, was detected by Western blot analysis and immunofluorescence microscopy of isolated sinus endothelial cells and tissue cryosections of the spleen. During the immunofluorescence microscopy of isolated endothelial cells, both caveolin-3 and caveolin-1 were found. In tissue cryosections of the spleen, caveolin-3, as well as caveolin-1 and -2, was present in the contours and cytoplasm of the cells. Immunogold electron microscopy of tissue cryosections revealed caveolin-3, -1, and -2 to be present in caveolae in the apical, lateral, and basal plasma membranes and some vesicular profiles in the cytoplasm of sinus endothelial cells. Furthermore, caveolin-3 was colocalized with caveolin-1 in the same caveolae in the apical, lateral, and basal plasma membranes. Stress fibers and tubulovesicular structures were situated in the vicinity of caveolae labeled with anti-caveolin-3, anti-caveolin-1, and anti-caveolin-2 antibodies. It is speculated that caveolae in sinus endothelial cells play an important role in the constriction of stress fibers.

Citations

Apr 28, 2005·Histochemistry and Cell Biology·Kiyoko Uehara
May 26, 2007·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Kayoko Nozawa-InoueTakeyasu Maeda
Feb 21, 2008·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Masahiro NiwanoTakeyasu Maeda
Jul 16, 2013·Molecular and Cellular Neurosciences·Ingrid R NiesmanHemal H Patel
Oct 11, 2005·Biochimica Et Biophysica Acta·Radu V Stan

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