Localization of CRMP5 mRNA by in situ hybridisation during development of the mouse forebrain

Neuroscience Letters
D McLaughlinD Karagogeos

Abstract

The expression of the collapse response mediator protein CRMP5 in the prenatal mouse is largely unknown. Evidence suggests that CRMP family members play important roles in neurite outgrowth, and CRMP5 is known to modulate outgrowth of processes in oligodendrocytes through signalling via neuropilin-1 and SemaA. Furthermore, CRMP family members function in axon regeneration after injury and are implicated in the early stages of Alzheimer's disease. Despite these findings relatively little is known about the specific roles these proteins play. The aim of the present study was to evaluate CRMP5 expression in the developing mouse forebrain using in situ hybridisation. Serial coronal sections of brain from E12.5 to E18.5 were analysed. We found highly specific patterns of expression which were restricted to the post-mitotic layers of both the ganglionic eminence and neocortex, and an additional domain of strong expression in the pyramidal layers of the hippocampus in all prenatal ages. Our results are therefore consistent with a role for CRMP5 in process extension. Interestingly, our results also revealed a temporal switch in high-expression levels from the ganglionic eminence to the cortex at a critical time during tangential cell m...Continue Reading

References

Jul 31, 2003·Journal of Neurochemistry·Yasuhiro SuzukiHiroshi Kiyama
Apr 19, 2005·The Journal of Comparative Neurology·Sylvie BretinChristian Gauchy
Nov 2, 2005·Molecular and Cellular Biology·Nariko ArimuraKozo Kaibuchi
Jan 3, 2006·Nature Neuroscience·Nicoletta KessarisWilliam D Richardson
Dec 22, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Naoya YamashitaYoshio Goshima

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.