Abstract
Recent reports indicate that the cytochrome P450 isozyme, CYP2D16, is expressed at high levels in the inner regions of the guinea pig adrenal cortex and may contribute to xenobiotic and/or steroid metabolism in the gland. In the present studies, immunohistochemical and in situ hybridization techniques were employed to definitively establish the localization of CYP2D16 within the adrenal cortex. In male guinea pigs of various ages, CYP2D16 protein and mRNA were highly localized to the zona reticularis (ZR); none was detectable in the zona fasciculata (ZF), zona glomerulosa (ZG) or the medulla. In contrast, the steroidogenic P450 isozyme, CYP17, was distributed throughout the ZF and ZR. From the earliest stages of development of the ZR, CYP2D16 staining was intense. As guinea pigs aged, the ZR progressively enlarged and comprised a proportionately greater amount of the cortex. At all ages, CYP2D16 was uniformly distributed throughout only the ZR. Coinciding with the age-related growth of the ZR and increase in adrenal CYP2D16 content was an increase in adrenal xenobiotic-metabolizing activity. The results establish that CYP2D16 has an intraadrenal localization that is unique among P450 isozymes, suggesting novel regulatory mechan...Continue Reading
References
Aug 1, 1975·The Journal of Endocrinology·A T FazekasW Gaus
Apr 1, 1991·The Journal of Endocrinology·T J McKenna, S K Cunningham
Jan 1, 1990·Journal of Biochemical Toxicology·E Hallberg
Sep 1, 1990·Endocrinology·V H Black
May 1, 1989·Endocrinology·V H BlackK O Martin
May 1, 1989·Endocrinology·V H BlackK O Martin
Nov 1, 1988·The Journal of Endocrinology·K ShinzawaS Takemori
May 1, 1985·The Journal of Endocrinology·G P VinsonB J Whitehouse
Jun 1, 1985·Molecular and Cellular Endocrinology·J B Adams
Apr 1, 1987·Analytical Biochemistry·T KronbachU A Meyer
Dec 1, 1987·Journal of Steroid Biochemistry·D A Staats, H D Colby
Nov 1, 1972·The American Journal of Anatomy·V H Black
Feb 1, 1967·Endocrinology·S Burstein, B R Bhavnani
Feb 1, 1983·Endocrinology·K O Martin, V H Black
Mar 7, 1983·Life Sciences·P I Eacho, H D Colby
May 1, 1982·Endocrinology·K O Martin, V H Black
Apr 26, 1995·Biochemical and Biophysical Research Communications·Q JiangH D Colby
Nov 28, 1993·Biochimica Et Biophysica Acta·N OhishiY Funae
Jun 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·H D ColbyH Purcell
Dec 1, 1952·Okajimas Folia Anatomica Japonica·T ITO
Nov 1, 1963·The Journal of Physiology·A B FAJER, M VOGT
Dec 18, 2007·Journal of Pediatric and Adolescent Gynecology·Shahla Nader
Dec 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·J B Schenkman
Citations
Feb 18, 1999·Molecular and Cellular Endocrinology·B B YuanH D Colby
Jul 6, 2001·Toxicology Letters·J TallkvistB Lönnerdal
May 3, 2011·PLoS Genetics·Guangju ZhaiHenri Wallaschofski