PMID: 8466933Apr 8, 1993Paper

Localization of Cys-344 on the (Ca(2+)-Mg(2+)-ATPase of sarcoplasmic reticulum using resonance energy transfer

Biochimica Et Biophysica Acta
A M MataA G Lee

Abstract

4-Bromomethyl-6,7-dimethoxy-coumarin labels the (Ca(2+)-Mg(2+)-ATPase of skeletal muscle sarcoplasmic reticulum at Cys-344. Resonance energy transfer has been used to measure the distance between this site and Lys-515 labelled with fluorescein isothiocyanate as about 37 A. The height of Cys-344 above the phospholipid/water interface has been measured by resonance energy transfer for the ATPase reconstituted into bilayers containing fluorescein-labelled phosphatidylethanolamine; the height was found to be about 45 A. None of these distances was found to alter on changing pH, or on addition of Mg2+, Ca2+ or vanadate. Quenching of the fluorescence of the coumarin-labelled ATPase with KI suggested that the fluorophore is not fully exposed on the ATPase.

References

Jan 1, 1979·Annual Review of Biochemistry·L de Meis, A L Vianna
Oct 11, 1979·Nature·R H Pearson, I Pascher
Jun 15, 1989·Biochemical and Biophysical Research Communications·I MatthewsJ M East
Dec 16, 1986·Biochimica Et Biophysica Acta·J A Teruel, J C Gómez-Fernández
Feb 26, 1987·Biochimica Et Biophysica Acta·C Gutierrez-MerinoA G Lee
Sep 15, 1981·Biochemistry·K P GhigginoD Phillips
Dec 1, 1980·Biophysical Journal·T G Dewey, G G Hammes

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Citations

Jan 1, 1995·Methods in Enzymology·P R Selvin
Oct 1, 1995·Bioscience Reports·A G LeeA P Starling
Jan 23, 2003·The Journal of Biological Chemistry·Man Liu, Andreas Barth
Feb 1, 1995·Current Opinion in Biotechnology·R M Clegg
May 6, 1996·Biochimica Et Biophysica Acta·J V MøllerM le Maire

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