Localization of diazepam-binding inhibitor-related peptides and peripheral type benzodiazepine receptors in the frog adrenal gland

Cell and Tissue Research
O LesouhaitierH Vaudry

Abstract

The adrenal gland of mammals contains high concentrations of peripheral-type benzodiazepine receptors (PBR) and diazepam-binding inhibitor (DBI), a polypeptide which acts as an endogenous ligand for PBR. The aim of the present study was to investigate the localization of DBI and PBR in the adrenal gland of the frog Rana ridibunda. Reverse transcription followed by polymerase chain reaction with specific primers for the frog DBI cDNA showed the presence of DBI mRNA in frog adrenal gland extracts. The cellular distribution of DBI and PBR was investigated using an antiserum against the octadecaneuropeptide DBI [33-50] (ODN) and antibodies against the 18-kDa isoquinoline binding protein subunit of PBR (IBP), respectively. ODN-like immunoreactivity was found in chromaffin cells and in Stilling s cells, but not in adrenocortical cells. IBP-like immunoreactivity was observed in chromaffin cells, in Stilling s cells and in a small proportion (11%) of steroid-secreting cells. The ODN- and IBP-immunoreactive materials were homogeneously distributed in the cytoplasm of chromaffin cells and concentrated at the periphery of large cytoplasmic vesicles in Stilling s cells. The proportion of ODN-positive Stilling s cells showed marked circannu...Continue Reading

Citations

Jul 18, 2001·The Journal of Steroid Biochemistry and Molecular Biology·F CartierC Delarue
May 5, 1999·Fundamental & Clinical Pharmacology·C J KenyonR Fraser
Jun 18, 1998·Annals of the New York Academy of Sciences·O LesouhaitierH Vaudry
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Jun 10, 2009·Frontiers in Neuroendocrinology·Jean Luc Do RegoHubert Vaudry
May 22, 2009·Annals of the New York Academy of Sciences·Jean-Luc Do RegoHubert Vaudry
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Jul 10, 2019·Pharmacology & Therapeutics·Marie-Christine TononJérôme Leprince

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