Localization of Drosophila neurons that contain choline acetyltransferase messenger RNA: an in situ hybridization study

The Journal of Comparative Neurology
R P BarberP M Salvaterra

Abstract

In situ hybridization with radiolabeled complementary RNA (cRNA) probes was used to determine the location of the messenger RNA (mRNA) encoding choline acetyltransferase (ChAT) in Drosophila nervous system. Areas in the cell-rich cortical regions of the cerebrum and optic lobes hybridized with substantial concentrations of the probe. This contrasted with the cell-sparse neuropil areas where no significant concentrations of probe were observed. Although most of the cortical regions were substantially labeled, there were regions within all of the areas where labeling was sparse or nonexistent. For example in the lamina, even though the monopolar cell layer appeared to be heavily labeled, there were some neuronal profiles that were not associated with the probe. Moreover, the epithelial glia that form an arch of cell profiles subjacent to the monopolar cells were not labeled, nor were amacrine neurons in the apex of the lamina near the external optic chiasma. The highest concentration of probe (approximately 140 grains/400 microns2) was observed in the laminar monopolar cell region and the cerebral cortical rind. The next most heavily labeled region (approximately 90 grains/400 microns2) occurred over cortical cells of the medulla...Continue Reading

References

Jan 1, 1976·Progress in Neurobiology·N Klemm
Feb 1, 1978·The Journal of General Physiology·A Klingman, R L Chappell
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M Brahic, A T Haase
Dec 1, 1977·Journal of Neurochemistry·B K Schmidt-NielsenL M Hall
Aug 1, 1987·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·R C Hardie
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·N ItohP M Salvaterra
Jan 1, 1986·Histochemistry·E P MeyerD R Nässel
Jun 13, 1966·Biochemical and Biophysical Research Communications·D T Denhardt
Oct 29, 1956·The Journal of Physiology·S E LEWIS, B N SMALLMAN
Dec 1, 1946·The Journal of Comparative Neurology·M E POWER

❮ Previous
Next ❯

Citations

Aug 1, 1992·Cellular and Molecular Neurobiology·R E Kennedy, J B Hutchins
Jan 1, 1991·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·M KawataY Sano
Mar 17, 2004·Brain Research. Gene Expression Patterns·P M Salvaterra, T Kitamoto
Nov 1, 1991·Journal of Neurochemistry·H SugiharaP M Salvaterra
Nov 1, 1990·Neurochemical Research·L A CarbiniP M Salvaterra
May 20, 1999·Microscopy Research and Technique·K Yasuyama, P M Salvaterra
Feb 1, 1993·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Toshihiro Kitamoto, Paul M Salvaterra
May 1, 1994·Journal of Neurochemistry·D Wu, L B Hersh
Nov 15, 2006·Learning & Memory·Jennifer E Mehren, Leslie C Griffith

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Forebrain- Circuits

Basal forebrain is a region in the brain important for production of acetylcholine and is the major cholinergic output of the CNS. Discover the latest research on circuits in the basal forebrain here.