Localization of elastin mRNA and TGF-beta1 in rat aorta and caudal artery as a function of age

Cell and Tissue Research
M SauvageM P Jacob

Abstract

Several in vitro studies have previously demonstrated that the addition of TGF-beta to aortic smooth muscle cells or skin fibroblasts stimulates elastin synthesis. It is not clear however whether, in vivo, TGF-beta participates in the regulation of elastin synthesis, especially in physiological conditions. The aim of our study was to explore the localization of elastin mRNA and TGF-beta1 in the rat thoracic aorta (an elastic artery) and caudal artery (a muscular artery). Elastin mRNA was localized by in situ hybridization and quantified using Northern blot analysis. TGF-beta1 was detected using immunohistochemistry. The study was carried out as a function of age (rats of 3, 10, 20, and 30 months). We observed that TGF-beta1 immunoreactivity is present predominantly, but not exclusively, at the sites of elastin synthesis as determined by elastin mRNA detection: in smooth muscle cells in the aorta and in endothelial cells in the caudal artery. The ability of exogenously added TGF-beta1 (0.001-10 ng/ml) to modulate the steady-state levels of elastin mRNA in primary cultures of endothelial cells, smooth muscle cells, and fibroblasts isolated from the thoracic aorta was also studied. At the highest concentration used, elastin mRNA l...Continue Reading

Citations

May 19, 2000·Experimental Gerontology·L Robert
Aug 26, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Jennifer L Long, Robert T Tranquillo
Jun 27, 2002·Seminars in Cancer Biology·Ladislas Robert
Jun 27, 2002·Seminars in Cancer Biology·Anna KadarLadislas Robert
Aug 31, 2006·Pediatric Research·Regina BökenkampMarco C Deruiter
Apr 1, 2014·Pathologie-biologie·M LannoyM-P Jacob
Aug 28, 2004·Journal of Cutaneous Pathology·Deanna GuerraPaola Ferrari
May 20, 2009·Journal of Tissue Engineering and Regenerative Medicine·Pankajakshan Divya, Lissy K Krishnan
Jun 27, 2013·Wiley Interdisciplinary Reviews. Developmental Biology·Ryan S UdanMary E Dickinson
Feb 9, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Francesco MassartMaria Luisa Brandi
Apr 10, 2012·International Journal of Cardiology·Alisson Martins-OliveiraJose E Tanus-Santos
Oct 2, 2007·Journal of Vascular Surgery·Jing WenElliot L Chaikof
Nov 23, 2011·Journal of Vascular Research·Olaf FritzeUlrich A Stock
May 26, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Eric DurandAntoine Lafont
Feb 20, 2016·Arteriosclerosis, Thrombosis, and Vascular Biology·Jie ZhangStephen F Vatner
Jan 9, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ping-Ping KuangRonald H Goldstein
May 26, 2020·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Ariel WangDaniela Valdez-Jasso
Apr 11, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Austin J CoccioloneJessica E Wagenseil
Sep 8, 2019·Matrix Biology : Journal of the International Society for Matrix Biology·Wassim FhayliGilles Faury
Apr 12, 2021·Mechanisms of Ageing and Development·Soo Hyuk KimMingyi Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved