PMID: 8591794Nov 1, 1995Paper

Localization of epidermal growth factor receptor in alveolar epithelium during human fetal lung development in vitro

Experimental Lung Research
J M KleinJ M Snyder

Abstract

Epidermal growth factor (EGF) enhances alveolar type II cell differentiation. In human fetal lung explants, EGF stimulates surfactant protein A (SP-A) synthesis. This effect may occur through a direct interaction of the ligand on EGF receptors located within distal pulmonary epithelium during alveolar type II cell differentiation. To determine if EGF receptor is present in alveolar epithelium, immunostaining for EGF receptor and in situ hybridization for EGF receptor mRNA were performed in human fetal lung explants undergoing alveolar type II cell differentiation in vitro. After 4 days in culture, EGF receptor immunostaining was present in alveolar epithelium from human fetal lung explants compared to minimal immunostaining in undifferentiated human fetal lung epithelium prior to culture. In situ hybridization revealed increased EGF receptor mRNA in differentiated type II cells from cultured explants, with minimal EGF receptor mRNA detected in undifferentiated epithelium from tissue prior to culture. Immunogold staining revealed EGF receptors on the cytoplasmic membranes of epithelial cells lining the prealveolar ducts in human fetal lung explants after 2 days in culture. Alveolar type II cell differentiation in vitro was confi...Continue Reading

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Citations

Apr 25, 2000·Indian Journal of Pediatrics·A R Kumar, J M Snyder
Sep 30, 2009·In Vitro Cellular & Developmental Biology. Animal·Richard C BaybuttWeiqun Wang
May 26, 2007·Applied Immunohistochemistry & Molecular Morphology : AIMM·Mercedes Cedeño-AriasTeresita Rodríguez
Jun 6, 2000·American Journal of Respiratory Cell and Molecular Biology·J M KleinJ M Snyder
Oct 17, 1998·Pediatric Pulmonology·H IJsselstijn, D Tibboel
Oct 12, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·David J FosterConnie C W Hsia
May 12, 1998·The American Journal of Physiology·J M KleinT A McCarthy

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