Localization of gelatinolytic activity can be detected in breast cancer tissues by film in situ zymography

Breast Cancer : the Journal of the Japanese Breast Cancer Society
H IwataS Kobayashi

Abstract

Extracellular matrix-degrading proteinases secreted by malignant tumor cells have been thought to play an essential role in the processes of invasion and metastasis. However, existence and localization of gelatinase activity in breast cancer tissues have not been clarified. We developed a novel film for highly reproducible detection and the localization of gelatinolytic activity. This film has a gelatin layer with a constant thickness 7 microm, and adequate crosslinking to control the speed of degradation by proteases. Cryosections of several breast cancer tissues were put on this gelatin film and incubated for 16 hrs at 37 degrees C. After staining with ponceau 3R dye, the digested area was evaluated under light microscopy. Digestion of gelatin was detected in more than 90%of breast cancer specimens, although it varied in degree and area for each case. In most cases, the gelatinolytic activity was located within cancer nests, and was not detected in stromal cells surrounding cancer cells. The gelatinolytic activity was inhibited by 1,10-phenanthroline, an inhibitor of matrix metalloproteinases (MMPs). In this study, the localization of net MMP activity was confirmed in breast cancer nest using film in situ zymography. Detailed...Continue Reading

References

Jul 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Z S GalisP Libby
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·A OkadaP Basset
Jan 1, 1993·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·H Birkedal-HansenJ A Engler
May 1, 1993·British Journal of Cancer·B DaviesF R Balkwill
Apr 1, 1996·Virchows Archiv : an International Journal of Pathology·M PoletteP Birembaut
Oct 1, 1996·American Journal of Respiratory and Critical Care Medicine·A PardoJ I Sznajder

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Citations

Oct 13, 2000·Breast Cancer : the Journal of the Japanese Breast Cancer Society·M NoguchiK Tsugawa
Feb 13, 2003·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Masahiro KajiYoshitaka Fujii
Dec 24, 2002·American Journal of Respiratory Cell and Molecular Biology·Jeffrey J Atkinson, Robert M Senior
Aug 25, 2006·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Akira FujiwaraNariaki Matsuura
Jul 8, 2003·International Journal of Cancer. Journal International Du Cancer·Naoto KamiyaHiroshi Ishikura
Dec 21, 2002·International Journal of Cancer. Journal International Du Cancer·Wulf SienelKlaus Pantel
May 20, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Wilma M Frederiks, Olaf R F Mook
Jun 16, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ryoichi NemoriYasunori Okada

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