Localization of genes for V+LDL plasma cholesterol levels on two diets in the opossum Monodelphis domestica.

Journal of Lipid Research
C M KammererPaul B Samollow

Abstract

Plasma cholesterol levels among individuals vary considerably in response to diet. However, the genes that influence this response are largely unknown. Non-HDL (V+LDL) cholesterol levels vary dramatically among gray, short-tailed opossums fed an atherogenic diet, and we previously reported that two quantitative trait loci (QTLs) influenced V+LDL cholesterol on two diets. We used hypothesis-free, genome-wide linkage analyses on data from 325 pedigreed opossums and located one QTL for V+LDL cholesterol on the basal diet on opossum chromosome 1q [logarithm of the odds (LOD) = 3.11, genomic P = 0.019] and another QTL for V+LDL on the atherogenic diet (i.e., high levels of cholesterol and fat) on chromosome 8 (LOD = 9.88, genomic P = 5 x 10(-9)). We then employed a novel strategy involving combined analyses of genomic resources, expression analysis, sequencing, and genotyping to identify candidate genes for the chromosome 8 QTL. A polymorphism in ABCB4 was strongly associated (P = 9 x 10(-14)) with the plasma V+LDL cholesterol concentrations on the high-cholesterol, high-fat diet. The results of this study indicate that genetic variation in ABCB4, or closely linked genes, is responsible for the dramatic differences among opossums in...Continue Reading

References

Jan 1, 1985·Cytogenetics and Cell Genetics·P M ConneallyR White
Jan 1, 1997·Genetic Epidemiology·S C HeathE M Wijsman
May 23, 1998·American Journal of Human Genetics·L Almasy, J Blangero
Aug 4, 1999·Current Opinion in Lipidology·R W Mahley, Y Huang
May 26, 2001·Annual Review of Nutrition·R M Krauss
Apr 18, 2002·Biochimica Et Biophysica Acta·Ronald Oude Elferink, Albert K Groen
Apr 30, 2003·The American Journal of Clinical Nutrition·Lindsey F MassonAlison Avenell
May 14, 2005·Current Opinion in Lipidology·Søren K Moestrup, Lars Bo Nielsen
Jun 3, 2005·Journal of Lipid Research·Silvia LangheimJonathan C Cohen
Aug 16, 2005·The Journal of Clinical Investigation·Luke J EngelkingMichael S Brown
Jul 4, 2006·Journal of the American Dietetic Association·Jose M Ordovas
Nov 18, 2006·Clinical Chemistry·José L González-SánchezManuel Serrano-Ríos
Mar 3, 2007·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Paul B SamollowCandace M Kammerer
May 15, 2007·Nature·Tarjei S MikkelsenKerstin Lindblad-Toh
Jan 15, 2008·Nature Genetics·Cristen J WillerGonçalo R Abecasis
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Dec 9, 2008·Nature Genetics·Sekar KathiresanL Adrienne Cupples
Feb 6, 2009·Atherosclerosis·Mark O GoodarziJerome I Rotter
Oct 9, 2009·Nature·Teri A ManolioPeter M Visscher
Apr 28, 2010·Seminars in Liver Disease·Anne Davit-SpraulEmmanuel Jacquemin
May 22, 2010·Journal of Lipid Research·Jeannie ChanJohn L VandeBerg

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Apr 19, 2013·Obesity·Nathalie Marissal-ArvyPierre Mormède

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