Localization of glucoamylase genes of Saccharomyces cerevisiae by pulsed field gel electrophoresis

Antonie van Leeuwenhoek
G R Bignell, I H Evans

Abstract

The chromosomal locations of four glucoamylase-specifying genes in the yeast Saccharomyces cerevisiae have been determined. Chromosomes were separated by pulsed field gel electrophoresis and blots were probed with radiolabelled STA2 and marker DNA from specific yeast chromosomes. The three genes encoding extracellular glucoamylases, STA1 (DEX2), STA2 (DEX1) and STA3 (DEX3) are located on chromosomes IV, II and XIV, respectively. SGA, specifying the sporulation-specific glucoamylase, was positioned on chromosome IX.

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Citations

Jun 1, 1996·Letters in Applied Microbiology·I Balogh, A Maráz
Jan 1, 1997·Critical Reviews in Biochemistry and Molecular Biology·M A VivierI S Pretorius
Mar 15, 2020·Applied Microbiology and Biotechnology·Kristoffer Krogerus, Brian Gibson

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