Sep 26, 1977

Localization of gonadotrophic hormones in the dog pituitary gland. A study using immunoenzyme histochemistry and chemical staining

Cell and Tissue Research
M F El Etreby, M R Fath El Bab


Using the immunoperoxidase technique and antisera to the specific beta (beta) subunits of FSH and LH1, selective immunochemical staining was localized mostly in the same cell type in the pars distalis and pars tuberalis of the dog pituitary gland. However, some cells were consistently shown to react solely with antisera to either LH beta of FSH beta. The cells stained for FSH beta were at least 1.5 times less numerous than those shown to contain LH beta. In the pars distalis of adult male dogs the immunoreactive gonadotrophs varied greatly in their relative proportion and were mostly shown to be much less numerous than in bitches in the anestrus phase of the sexual cycle. These cells were found to be positive to aldehyde fuchsin, alcian blue, periodic acid-Schiff (PAS) and aniline blue. The performic acid-alcian blue (pH 0.2)-PAS-orange G procedure stained the FSH/LH cells blue or turquoise, demonstrating TSH cells (blue-purple), ACTH/MSH cells (red-purple) and PRL cells (orange-red). The FSH/LH cells were further differentiated from other functional cell types of the pars distalis on the basis of their typical cytological features, intraglandular distribution and by immunochemical double staining. These observations support t...Continue Reading

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Mentioned in this Paper

Establishment and Maintenance of Localization
Female dog
POMC wt Allele
Immune Sera
Water soluble aniline blue stain
Follicle Stimulating Hormone, beta Subunit
Cell Differentiation Process

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