PMID: 9432001Feb 7, 1998Paper

Localization of heparin binding activity in recombinant laminin G domain

European Journal of Biochemistry
U SungPeter D Yurchenco

Abstract

Basement membrane laminin (laminin-1) is a multidomain glycoprotein that interacts with itself, heparin and cells. The interaction with heparin/heparan sulfate proteglycans is thought to be important for the architectural formation of basement membranes and adhesion to cells. The major heparin binding site has been known to reside in the long arm globular domain (G domain). The G domain is in turn subdivided into five subdomains (G1-G5). In order to localize the heparin binding regions further, recombinant G domains (rG and rG5) were expressed in Sf9 insect cells using baculovirus expression vector. By the limited proteolysis of recombinant G domains followed by either heparin affinity HPLC or overlay with radiolabeled heparin, the relative affinity of each subdomain to heparin was assigned as G1>G2 = G4>G5>G3, such that G1 bound strongly and G3 not at all. Since the activity in G1-G3 is cryptic in intact laminin long arm [Sung, U., O'Rear, J. J. & Yurchenco, P. D. (1993) J. Cell Biol. 123, 1255-1268], the active heparin binding site of G domain appears to be located in G4 and proximal G5.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M K Lee, A D Lander
Mar 8, 1991·Cell·E Ruoslahti, Y Yamaguchi
Mar 1, 1990·The Journal of Cell Biology·J C Schittny, P D Yurchenco
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D W SpeicherV T Marchesi
Mar 1, 1982·European Journal of Biochemistry·U OttR Timpl

❮ Previous
Next ❯

Citations

Jun 8, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H Colognato, P D Yurchenco
Apr 26, 2007·Nature Reviews. Cancer·M Peter Marinkovich
Aug 13, 2005·Journal of Molecular Biology·Anirban Bhaduri, R Sowdhamini
Mar 7, 2008·Cellular & Molecular Biology Letters·Xiu-Sheng HeZhu-Chu Chen
Apr 27, 2000·Mechanisms of Development·S KuriyamaT Kinoshita
Sep 2, 2011·The Journal of Infectious Diseases·Teresia HallströmKristian Riesbeck
Oct 12, 2010·Current Eye Research·Sayon RoyEkaterina Beglova
Aug 8, 1998·The Journal of Biological Chemistry·N ParthasarathyB Mulloy
Jul 13, 2000·The Journal of Biological Chemistry·H YamaguchiY Kitagawa
May 9, 2000·The Journal of Biological Chemistry·P K NielsenY Yamada
Oct 29, 2003·Biochemistry·Nobuharu SuzukiMotoyoshi Nomizu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.