PMID: 9525659Apr 3, 1998Paper

Localization of human cytomegalovirus structural proteins to the nuclear matrix of infected human fibroblasts

Journal of Virology
V SanchezWilliam J Britt

Abstract

The intranuclear assembly of herpesvirus subviral particles remains an incompletely understood process. Previous studies have described the nuclear localization of capsid and tegument proteins as well as intranuclear tegumentation of capsid-like particles. The temporally and spatially regulated replication of viral DNA suggests that assembly may also be regulated by compartmentalization of structural proteins. We have investigated the intranuclear location of several structural and nonstructural proteins of human cytomegalovirus (HCMV). Tegument components including pp65 (ppUL83) and ppUL69 and capsid components including the major capsid protein (pUL86) and the small capsid protein (pUL48/49) were retained within the nuclear matrix (NM), whereas the immediate-early regulatory proteins IE-1 and IE-2 were present in the soluble nuclear fraction. The association of pp65 with the NM resisted washes with 1 M guanidine hydrochloride, and direct binding to the NM could be demonstrated by far-Western blotting. Furthermore, pp65 exhibited accumulation along the nuclear periphery and in far-Western analysis bound to proteins which comigrated with proteins of the size of nuclear lamins. A direct interaction between pp65 and lamins was de...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·A E GoldmanR D Goldman
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·I GreenfieldM Stanley
Dec 1, 1990·Journal of Virology·E RoffmanN Frenkel
Mar 1, 1990·The Journal of Cell Biology·D C HeS Penman
Jan 1, 1988·Annual Review of Cell Biology·L Gerace, B Burke
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E G Fey, S Penman
Jan 1, 1987·Annual Review of Biochemistry·J W Newport, D J Forbes
Jun 1, 1986·Virus Research·G KhittooR Simard
Jan 1, 1986·Annual Review of Cell Biology·C Dingwall, R A Laskey
Apr 1, 1987·Journal of Virology·Z H ZhaiS Penman
Sep 1, 1982·Virology·V Bibor-HardyR Simard
Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·B GuoG S Stein
Oct 1, 1995·The Journal of Cell Biology·T CarvalhoA Dejean
May 1, 1994·Journal of Cellular Biochemistry·K J Pienta, C N Hoover
May 1, 1994·Journal of Cellular Biochemistry·R H Getzenberg
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·M A ManciniW H Lee
Jul 1, 1993·The Journal of Cell Biology·N Chaudhary, J C Courvalin
Jan 1, 1995·International Review of Cytology·J A NickersonS Penman

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Citations

Jan 30, 2004·American Journal of Physiology. Cell Physiology·Lilia M MaglovaJohn M Russell
Jul 9, 2009·Journal of Cellular Biochemistry·Maria-Cristina ArcangelettiCarlo Chezzi
Mar 29, 2012·Immunologic Research·Vanessa NoriegaDomenico Tortorella
Nov 5, 2002·Journal of Virology·Catherine A Blankenship, Thomas Shenk
Nov 23, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ann-Charlotte IversenChris A Benedict
Apr 19, 2002·The Journal of General Virology·P Dal MonteM P Landini
Apr 12, 2005·Nature Immunology·Tal I ArnonOfer Mandelboim
Jan 14, 2010·Reviews in Medical Virology·Chung-Pei Lee, Mei-Ru Chen
Jun 25, 2005·The Journal of Biological Chemistry·Manfred MarschallThomas Stamminger
Dec 22, 2000·Journal of Molecular Biology·M Kenzelmann, K Mühlemann

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