PMID: 7546906Jul 1, 1995Paper

Localization of human immunodeficiency virus Rev in transfected and virus-infected cells

AIDS Research and Human Retroviruses
L LuznikF Wong-Staal

Abstract

The rev gene product of human immunodeficiency virus (HIV) is obligatory for viral replication. Rev interacts specifically with a structured RNA sequence within the viral genome termed the REV response element (RRE). Although the importance of Rev for the expression of viral proteins is well documented, its functional mechanism remains unresolved. Previous studies identified Rev in the absence of RRE to be a nuclear protein localized primarily within the nucleoli. To extend our understanding of the role of Rev in viral replication, immunolocalization studies of Rev and other nuclear components were carried out in transfected cells expressing both the Rev protein and RRE-containing mRNA and in cells infected with HIV. In both types of cells, Rev-like immunoreactivity was distributed both in the nucleoplasm and cytoplasm. Within the nucleus, Rev immunoreactivity was not evenly distributed but was present within focal concentrations. In transfected cells that were double labeled for Rev and SC-35, which labels a known component of spliceosomes, the foci of Rev labeling were distinct from the "speckles" labeled by SC-35, although Rev foci and speckles were often juxtaposed. In addition, morphological changes in the three-dimensiona...Continue Reading

References

Jan 1, 1991·Annual Review of Biochemistry·Y N Vaishnav, F Wong-Staal
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D L Spector
Feb 1, 1990·Molecular and Cellular Biology·R A Katz, A M Skalka
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S DaeflerF Wong-Staal
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·X B LuM L Hammarskjöld
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J B LawrenceR H Singer
Dec 7, 1989·Nature·M L Zapp, M R Green
Jan 24, 1985·Nature·L RatnerK Baumeister
May 1, 1989·Journal of Virology·M L HammarskjöldD Rekosh
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B K FelberG N Pavlakis
Nov 1, 1987·The Journal of Cell Biology·T E MartinP G Spear
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D RekoshH Wigzell
Nov 1, 1988·Molecular and Cellular Biology·S Arrigo, K Beemon
Jan 1, 1986·The Journal of Cell Biology·U NymanN R Ringertz
Jun 10, 1988·Science·H Varmus
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·G Blobel
Nov 5, 1993·Cell·M Rosbash, R H Singer
Jul 1, 1994·Genes & Development·B E Meyer, M H Malim
Feb 1, 1994·The Journal of Cell Biology·R T O'KeefeD L Spector
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R TalbottF Wong-Staal

❮ Previous
Next ❯

Citations

Apr 1, 1996·Journal of Biomedical Science·Y. XuF. Wong-Staal
Apr 5, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Hoshang J UnwallaJohn J Rossi
Nov 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Virginie W GautierWilliam W Hall
Oct 12, 2002·Proceedings of the National Academy of Sciences of the United States of America·Alessandro MichienziJohn J Rossi
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·A MichienziJ J Rossi
Jun 19, 2008·BMC Cell Biology·Donatella PontiFranca Gigliani
May 6, 1998·Virology·P Corbeau, F Wong-Staal
Feb 1, 2012·PLoS Genetics·Michael P HeatonWilliam W Laegreid

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.