PMID: 3663609Jul 14, 1987

Localization of hydrophobic ions in phospholipid bilayers using 1H nuclear Overhauser effect spectroscopy

Biochemistry
J F EllenaD S Cafiso

Abstract

The binding location for the hydrophobic ions tetraphenylphosphonium (TPP+) and tetraphenylboron (TPB-) was studied in sonicated phosphatidylcholine (PC) vesicles by measuring time-dependent and steady-state intermolecular 1H nuclear Overhauser effects (NOE's). Intermolecular cross-relaxation was also investigated by two-dimensional NOE spectroscopy. Information on the distance and order parameter dependence of the NOE's was obtained from a simple simulation of the NOE's in the alkyl chain region. Taken together, the NOE data and the simulation provide strong evidence that TPB- and TPP+, at low concentrations (less than or equal to 10 mol%), are localized in the alkyl chain region of the bilayer. At these lower concentrations of TPP+ or TPB-, no significant effect on lipid 13C T1 or T2 relaxation rates is detected. The proposed location is consistent with the expected free energy profiles for hydrophobic ions and with the carbonyl oxygens or interfacial water as the source of the membrane dipole potential. At higher ion/lipid ratios (greater than or equal to 20 mol%), TPB-/lipid NOE's increase. This results from a specific association of TPB- with the choline head group.

References

Apr 1, 1976·Canadian Journal of Biochemistry·N G SeidahR Laprade
Jan 1, 1978·Biophysical Journal·O S AndersenS McLaughlin
Jan 19, 1979·Biochimica Et Biophysica Acta·B A LevineR J Williams
Jan 1, 1969·Biochimica Et Biophysica Acta·O H Le Blanc
Jan 1, 1981·Annual Review of Biophysics and Bioengineering·D S Cafiso, W L Hubbell
Nov 1, 1981·Quarterly Reviews of Biophysics·P LäugerW Brock
May 22, 1984·Biochemistry·D J SiminovitchK R Jeffrey
Sep 1, 1982·Biophysical Journal·D S Cafiso, W L Hubbell
Feb 1, 1980·Quarterly Reviews of Biophysics·J Seelig, A Seelig
Dec 2, 1980·Biochimica Et Biophysica Acta·E E BurnellB de Kruijff
Jan 1, 1965·Journal of the American Oil Chemists' Society·W S SINGLETONJ L WHITE
Sep 1, 1971·The Journal of Membrane Biology·B KettererP Läuger
Dec 1, 1970·The Journal of Membrane Biology·B Neumcke, P Läuger

Citations

Jun 15, 2007·European Biophysics Journal : EBJ·Grégory Da CostaArnaud Bondon
Oct 8, 1990·Biochimica Et Biophysica Acta·G Cevc
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S A Simon, T J McIntosh
May 1, 1989·European Journal of Biochemistry·F SolerJ C Gómez-Fernández
Jan 18, 1993·Biochimica Et Biophysica Acta·G L JendrasiakA A Ribeiro
Apr 19, 2005·Magnetic Resonance Imaging·R G Bryant, Jean-Pierre Korb
Dec 14, 2007·Physical Chemistry Chemical Physics : PCCP·Martin P Boland, David A Middleton
Nov 30, 2007·The Journal of Biological Chemistry·Michael E GreenbergStanley L Hazen

Related Concepts

tetraphenylphosphonium bromide
Borides
Lipid Bilayers
In Vivo NMR Spectroscopy
Onium Compounds
Organopyrophosphorus Compounds
Phosphatidylcholines
Tetraphenylborate, Barium Salt (2: 1)

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.