Localization of laminin proteins during early follicular development in pig and rabbit ovaries

Journal of Reproduction and Fertility
V H LeeB S Dunbar

Abstract

Laminin, a major component of the basal lamina, is known to be important in the differentiation of epithelial cells. The outer granulosa cell layer of ovarian follicles is attached to a basal lamina surrounding the follicle and it has been demonstrated that proteins of the basal lamina can alter the steroidogenic capacity and cytoskeletal composition of mature granulosa cells. The present studies were carried out to evaluate the developmental expression of laminin proteins in the basal lamina of ovarian follicles during early stages of granulosa cell differentiation. Ovaries from sexually immature pigs (0-20 weeks of age) and rabbits (0-12 weeks of age) were used for this study because formation of primordial follicles and initiation of follicular growth occur partially or completely postnatally. Specific antibodies were made against laminin beta 1/gamma 1 chains using protein purified by high resolution two-dimensional polyacrylmide gel electrophoresis. These antibodies were used in immunohistochemical localization studies to demonstrate that laminin is associated with the basal lamina surrounding egg clusters and their connections to the ovarian surface epithelium and ovarian rete during early ovarian development. In addition...Continue Reading

Citations

Jul 11, 2006·Cell and Tissue Research·Karla J HuttMichael K Holland
Jul 27, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·C M MeltonV H Lee
Mar 1, 1996·American Journal of Reproductive Immunology : AJRI·S M SkinnerB S Dunbar
Jan 21, 2014·BMC Genomics·Nicholas HatzirodosRaymond J Rodgers
Jul 10, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·George B JohnDiego H Castrillon
May 23, 2014·Journal of Developmental Origins of Health and Disease·Pauline LéveilléPascale Chavatte-Palmer
Feb 11, 2012·Maturitas·Jung-A PyunKyuBum Kwack
Nov 19, 2015·Frontiers in Oncology·Angela ChoEmily K Colvin
Dec 17, 2009·Journal of Morphology·Brandon C MooreLouis J Guillette
May 11, 1999·Differentiation; Research in Biological Diversity·K FröjdmanI Virtanen
Nov 7, 2002·Molecular Reproduction and Development·Katie L KennedyVaughan H Lee
Jun 16, 2001·Endocrine Reviews·S F PalterE Y Adashi
Aug 6, 2002·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·C Ioannides
Jun 22, 2000·Biology of Reproduction·A RendonV H Lee
Oct 2, 2002·Molecular and Cellular Endocrinology·Cole DicksonShafiq A Khan
Sep 7, 2017·Journal of Proteome Research·Kelley M HeffnerMichael J Betenbaugh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.