Localization of leptin receptor immunoreactivity in the lean and obese Zucker rat brain

Brain Research
D O YarnellP Tso

Abstract

Leptin, a product of the obese (ob) gene, is secreted by adipocytes and appears to act as a hormone to regulate food intake, metabolism and body weight. Subcutaneous administration of leptin causes reductions in food intake and body and fat-depot weights in both lean and genetically obese (ob/ob) mice, and leptin infusion into the lateral cerebral ventricles decreases feeding with short latency, suggesting a central site of action. A gene defect in the Zucker obese rat causes an amino acid substitution in the leptin receptor and reduced leptin binding at the cell surface. An antiserum to a portion of the mouse leptin receptor (AA 877-894) located within the intracellular domain was used to label Zucker lean (Fa/?) and obese (fa/fa) rat brain sections. At optimal dilution (1:8000), only cells in the basal forebrain, preoptic area, hypothalamus and brainstem were moderately or intensely labeled. The most intensely-labeled nuclei, the anterior commissural, magnocellular paraventricular, supraoptic, circularis in the anterior hypothalamus and fornical in the lateral hypothalamus contain large neurons that synthesize and secrete vasopressin or oxytocin and their respective neurophysins. Diminished leptin transport into the central n...Continue Reading

Citations

Feb 20, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D G BaskinJ P Kochan
Apr 24, 2012·Journal of Obesity·Fabio PanarielloVincenzo De Luca
Jun 20, 2001·Journal of Neuroendocrinology·M L OvesjöB Meister
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Oct 7, 2003·Anatomical Science International·Hisayuki FunahashiSeiji Shioda
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