Localization of Mel1b melatonin receptor-like immunoreactivity in ocular tissues of Xenopus laevis

Experimental Eye Research
A F WiechmannJody A Summers Rada

Abstract

The circadian signaling molecule, melatonin, is produced by pinealocytes and retinal photoreceptors. In the retina, melatonin is thought to diffuse into the inner retina to act as a paracrine signal of darkness by binding to specific receptors in retinal neurons. The retinal cell locations of the Mel1a and Mel1c melatonin receptor types have been reported, but the localization of the Mel1b receptor, which is the most highly expressed melatonin receptor type in the retina, is unknown. To determine the cellular distribution of Mel1b melatonin receptor protein in the Xenopus laevis retina and other ocular tissues, polyclonal antibodies were raised against a peptide fragment of the X. laevis Mel1b receptor. Western blot analysis of several ocular tissues revealed the presence of one or more immunoreactive bands in the sclera, cornea, lens, retinal pigment epithelium (RPE)/choroid, and neural retina. In the neural retina, the major immunoreactive bands displayed electrophoretic mobilities corresponding to approximately 35, 42, 45, and 80 Kd. Sections of X. laevis eyes were analyzed by immunocytochemistry and confocal microscopy, in combination with antibodies against the Mel1a melatonin receptor, a rod photoreceptor-specific protein...Continue Reading

References

Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·T EbisawaS M Reppert
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·S M ReppertJ F Gusella
Apr 1, 1993·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·S Weiss, F Schaeffel
Jan 8, 1999·Brain Research. Molecular Brain Research·A F WiechmannD M Defoe
Aug 18, 1999·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·D L NicklaJ Wallman
Jul 18, 2001·Brain Research. Molecular Brain Research·A F Wiechmann, A R Smith
Jul 12, 2002·Cell and Tissue Research·Charlotte von GallDavid R Weaver
Aug 20, 2002·The British Journal of Ophthalmology·P MeyerE Savaskan
Sep 6, 2003·Frontiers in Bioscience : a Journal and Virtual Library·Margarita L DubocovichMonica I Masana

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Citations

Aug 3, 2005·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Esther IsornaAngel Luis Alonso-Gómez
Aug 19, 2007·Cell and Tissue Research·Virginie Dinet, Horst-Werner Korf
Oct 22, 2005·The Journal of Physiology·Hai HuangXiong-Li Yang
Sep 19, 2012·Progress in Retinal and Eye Research·Hai HuangXiong-Li Yang
Apr 5, 2008·The Journal of Physiology·Yong PingXiong-Li Yang
Mar 5, 2008·Progress in Retinal and Eye Research·Allan F Wiechmann, Jody A Summers
Jan 19, 2007·Pharmacology & Therapeutics·Pilar Alarma-Estrany, Jesús Pintor
Dec 19, 2006·Experimental Eye Research·Per O LundmarkR E Rosenstein
May 5, 2009·Molecular and Cellular Endocrinology·Michał A ZmijewskiAndrzej T Slominski
Jan 3, 2007·Journal of Pineal Research·Masanori T ItohKimiya Shimizu
Jan 31, 2006·Journal of Pineal Research·Andrew W SiuRussel J Reiter
Jul 22, 2008·Journal of Pineal Research·Hülya Cevik Aras, Jörgen Ekström
May 25, 2010·Journal of Pineal Research·Ruth E RosensteinDaniel P Cardinali
Apr 7, 2015·Medical Hypotheses·Peter H Frederikse, Chinnaswamy Kasinathan
Jan 11, 2012·Journal of Neuroscience Methods·Aleksandr SaghyanSabina Hrabetova
Jun 29, 2017·Journal of Pineal Research·Almudena CrookeJesús Pintor
Oct 9, 2007·Neuroreport·Min ZhangXiong-Li Yang
Jan 1, 2015·Journal of Circadian Rhythms·Nermien E Waly, Richard Hallworth
Sep 29, 2019·Progress in Retinal and Eye Research·Hanan Awad AlkoziJesus Pintor
Mar 7, 2021·Biomolecules·Alejandro Martínez-ÁguilaGonzalo Carracedo

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