PMID: 12759455May 22, 2003Paper

Localization of MHC class II/human cartilage glycoprotein-39 complexes in synovia of rheumatoid arthritis patients using complex-specific monoclonal antibodies

The Journal of Immunology : Official Journal of the American Association of Immunologists
P G SteenbakkersA M Boots

Abstract

Recently human cartilage gp-39 (HC gp-39) was identified as a candidate autoantigen in rheumatoid arthritis (RA). To further investigate the relevance of this Ag in RA, we have generated a set of five mAbs to a combination epitope of complexes of HC gp-39(263-275) and the RA-associated DR alpha beta 1*0401 HLA class II molecules. FACS studies revealed that these mAb recognize specific complexes on homozygous DR alpha beta 1*0401-positive B lymphoblastoid cells pulsed with HC gp-39(263-275). The best mAb, 12A, was further characterized using a set of irrelevant DR alpha beta 1*0401-binding peptides and truncated/elongated versions of HC gp-39(263-275) itself. The minimal epitope recognized in combination with DR alpha beta 1*0401 was HC gp-39(263-273). Peptides not encompassing HC gp-39(263-273) were not recognized. Three of five mAb were able to inhibit (up to 90%) the response of HC gp-39(263-275)-specific DR alpha beta 1*0401-restricted T cell hybridomas to peptide-pulsed APC or purified complexes. Using mAb 12A, we have been able to identify and localize dendritic cells that present DR alpha beta 1*0401/HC gp-39(263-275) complexes in synovial tissue of DR alpha beta 1*0401-positive RA patients, indicating local presentation ...Continue Reading

References

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Citations

Nov 6, 2004·Current Rheumatology Reports·Gijs Teklenburg, Salvatore Albani
Nov 13, 2008·Critical Reviews in Clinical Laboratory Sciences·Frederick D Coffman
Sep 14, 2012·Arthritis and Rheumatism·Nataliya YeremenkoDominique Baeten
Nov 5, 2014·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Koichi Ueno
Mar 1, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Heidi S SchultzSvetlana Panina
Sep 24, 2019·Antibodies·Lene Støkken HøydahlGeir Åge Løset
May 2, 2007·Nature Clinical Practice. Rheumatology·Danielle M Gerlag, Paul P Tak
Nov 6, 2007·Biological & Pharmaceutical Bulletin·Emi OhkiKoichi Ueno
Oct 5, 2011·Arthritis and Rheumatism·Troy NoordenbosDominique Baeten
Oct 3, 2009·Nature Reviews. Rheumatology·Shaukat KhanNina Bhardwaj
Sep 12, 2015·Rheumatology·Marleen G van de Sande, Dominique L Baeten
Jan 24, 2018·Annals of the Rheumatic Diseases·Catriona T PrendergastRobert A Benson
Sep 16, 2020·Signal Transduction and Targeted Therapy·Ting ZhaoQiang You
Sep 23, 2006·Journal of Cellular and Molecular Medicine·Julia KzhyshkowskaS Goerdt
Nov 30, 2013·Rheumatology·Nataliya YeremenkoDominique Baeten
Oct 2, 2009·Future Oncology·Julia S JohansenBenny V Jensen

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