PMID: 7538415Feb 1, 1995Paper

Localization of multiple functional domains on human PECAM-1 (CD31) by monoclonal antibody epitope mapping

Cell Adhesion and Communication
H C YanSteven M Albelda

Abstract

PECAM-1, a cell adhesion molecule of the immunoglobulin gene (Ig) superfamily, has been implicated in white cell transmigration, integrin activation on lymphocytes, and cell-cell adhesion. The purpose of this investigation was to identify specific regions of the PECAM-1 extracellular domain mediating these functions by identifying the location of epitopes of bioactive anti-PECAM-1 monoclonal antibodies. The binding regions of mAbs important in PECAM-1-mediated leukocyte transmigration (Hec 7.2 and 3D2) were mapped to N-terminal Ig-like domains. The epitopes of monoclonal antibodies that activated integrin function on lymphocytes were dispersed over the entire extracellular region, but those that had the strongest activating effect were preferentially localized to the N-terminus of the molecule. The binding regions of mAbs that blocked PECAM-1-mediated heterophilic L-cell aggregation were located either in Ig-like domain 2 (NIH31.4) or Ig-like domain 6 (4G6 and 1.2). Site-directed mutagenesis further pinpointed the epitope of the 4G6 mAb to a hexapeptide, CAVNEG, within Ig-like domain 6. These results demonstrate that PECAM-1 contains multiple functional domains. Regions within N-terminal Ig-like domains appear to be required fo...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Aug 15, 1992·Analytical Biochemistry·J Yuan, P S Low
Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S ChenM I Greene
Mar 1, 1992·Human Immunology·K T Hogan, S L Brown
Nov 1, 1992·Journal of Cellular Physiology·L A SchimmentiS M Albelda
May 1, 1992·The Journal of Experimental Medicine·W A MullerS M Albelda
Jan 1, 1992·Analytical Biochemistry·W P Deng, J A Nickoloff
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J V FecondoA W Boyd
May 18, 1990·Cell·W G LaverS J Smith-Gill
Mar 1, 1991·American Journal of Respiratory Cell and Molecular Biology·S M Albelda
Apr 1, 1990·The Journal of Cell Biology·S M AlbeldaC A Buck
Jan 1, 1988·Annual Review of Immunology·A F Williams, A N Barclay
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Jul 1, 1993·Molecular and Cellular Biology·J ChenA J Levine
Sep 1, 1993·The Journal of Cell Biology·D T ShihC A Buck
Mar 1, 1994·The Journal of Experimental Medicine·S BogenW A Muller
Aug 1, 1993·The Journal of Experimental Medicine·W A MullerD M Phillips
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y Xie, W A Muller

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Citations

Aug 6, 2002·Trends in Cardiovascular Medicine·Jonathan M Gibbins
Feb 25, 2003·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Teresa J BohlmeyerM Benjamin Perryman
Jan 1, 1997·The Journal of Clinical Investigation·P J Newman
Mar 22, 2000·The Journal of Cell Biology·K A ReedquistJ L Bos
Apr 3, 2002·The Journal of Cell Biology·Guangju JiMichael I Kotlikoff
Jun 20, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Silvia Muro
Nov 4, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Vladimir V ShuvaevVladimir R Muzykantov
Sep 11, 2009·Journal of Thrombosis and Haemostasis : JTH·E OrlowskiD E Jackson
Jul 14, 2004·Biochemical and Biophysical Research Communications·David A KaufmanPeter F Davies
Nov 3, 2005·Biochimica Et Biophysica Acta·Curzio RüeggGian Carlo Alghisi
Jul 27, 2014·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Fumin ChangLewis Romer
Jan 11, 2007·Tissue Antigens·F-M Robbins, R J Hartzman
May 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C D O'BrienS M Albelda
Feb 7, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A ScherpereelV R Muzykantov
Jun 21, 2007·The Journal of Biological Chemistry·Ulrich J H SachsSentot Santoso
Apr 20, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Giulia FornasaGiuseppina Caligiuri
Jan 24, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christopher J KuckleburgPeter J Newman
Jun 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·R D ThompsonS Nourshargh
Dec 25, 2015·Blood·Cathy PaddockJieqing Zhu

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