PMID: 407Dec 1, 1975

Localization of NADH oxidase on the surface of human polymorphonuclear leukocytes by a new cytochemical method

The Journal of Cell Biology
R T BriggsM J Karnovsky


The ultrastructural localization of NADH oxidase, a possible enzyme in the increased oxidative activity of polymorphonuclear leukocytes (PMN) during phagocytosis, was studied. A new cytochemical technique for the localization of H2O2, a product of NADH oxidase activity, was developed. Cerous ions, in the presence of peroxide, form an electron-dense precipitate. Resting and phagocytically stimulated PMN were exposed to cerous ions at pH 7.5 to demonstrate sites of NADH-dependent, cyanide-insensitive H2O2 production. Resting PMN exhibites slight activity on the plasma membrane; phagocytizing PMN had extensive deposits of reaction product localized within the phagosome and on the plasma membrane. Peroxide involvement was demonstrated by the inhibitory effect of catalase on cerium precipitation; the surface localization of the enzyme responsible was confirmed by using nonpenetrating inhibitors of enzymatic activity. A correlative study was performed with an NADH-dependent, tetrazolium-reduction system. As with cerium, formazan deposition on the surface of the cell was NADH dependent, cyanide insensitive, and stimulated by phagocytosis. Superoxide dismutase did not inhibit tetrazolium reduction, as observed cytochemically, indicatin...Continue Reading


Feb 17, 1975·Biochemical and Biophysical Research Communications·K Takanaka, P J O'Brien
Jan 1, 1975·The Journal of Cell Biology·R T BriggsM J Karnovsky
Jul 1, 1971·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K L SimsF E Bloom
Aug 1, 1971·The Journal of Clinical Investigation·T P StosselM Vaughan
Aug 6, 1973·Biochemical and Biophysical Research Communications·P PatriarcaF Rossi
Nov 23, 1974·Lancet·A W Segal
Mar 15, 1974·Science·J W DePierre, M J Karnovsky
Aug 1, 1970·Life Sciences. Pt. 1: Physiology and Pharmacology·P PatriarcaF Rossi
Mar 1, 1973·The Journal of Clinical Investigation·B M BabiorJ T Curnutte
Mar 14, 1974·The New England Journal of Medicine·J T CurnutteB M Babior
Jun 1, 1974·The Journal of Clinical Investigation·J T Curnutte, B M Babior
May 2, 1968·The New England Journal of Medicine·R L Baehner, D G Nathan
Apr 6, 1971·Biochimica Et Biophysica Acta·G L Mandell, G W Sullivan
Jan 1, 1971·Annual Review of Medicine·Seymour J Klebanoff
Oct 1, 1974·The Journal of Clinical Investigation·M L Salin, J M McCord
Jan 31, 1974·The New England Journal of Medicine·D G Nathan
Jan 1, 1969·The Journal of Cell Biology·R H MichellM L Karnovsky
Dec 1, 1967·The Journal of Experimental Medicine·Seymour J Klebanoff
Jul 1, 1953·The Journal of Pathology and Bacteriology·H HARRIS
Jun 1, 1963·The Journal of Cell Biology·R ORENM L Karnovsky
Oct 17, 1964·Nature·R H CAGAN, M L Karnovsky
May 1, 1965·The Journal of Cell Biology·J H VENABLE, R COGGESHALL
Mar 1, 1965·The Journal of General Physiology·J VANSTEVENINCKA ROTHSTEIN


Jan 1, 1985·Histochemistry·L Rechardt, H Hervonen
Jan 1, 1986·Histochemistry·T OkadaM L Karnovsky
Dec 1, 1981·Journal of Bioenergetics and Biomembranes·T RamasarmaF L Crane
Jan 1, 1981·The Histochemical Journal·M J KarnovskyM L Karnovsky
Jan 1, 1982·The Histochemical Journal·T FujimotoK Ogawa
Mar 1, 1995·Histochemistry and Cell Biology·J M Robinson, J A Badwey
Jul 4, 2008·Histochemistry and Cell Biology·J M Robinson
Jan 1, 1983·The International Journal of Biochemistry·M J Connock, N J Temple
Jan 1, 1985·Comparative Immunology, Microbiology and Infectious Diseases·A Ryter
Nov 1, 1981·Molecular and Cellular Endocrinology·R Ekholm
Jan 5, 2000·Journal of Immunological Methods·J M RobinsonT Takizawa
Dec 1, 1993·Acta histochemica·G Nakos, R Gossrau
May 1, 1993·Acta histochemica·I H Straatsburg, R Gossrau
Oct 17, 2001·Developmental and Comparative Immunology·N F NeumannMiodrag Belosevic
Feb 28, 2002·Free Radical Biology & Medicine·Minjie ZhangKiyohisa Sekizawa
Jan 1, 1997·Free Radical Biology & Medicine·W M Frederiks, K S Bosch
Jan 22, 1998·Free Radical Biology & Medicine·E Ann EllisG A Lutty
Aug 13, 2004·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Maria Fernanda Reis Gavazzoni DiasW de Souza
Jul 1, 2002·Molecular Plant Pathology·Alexander SchoutenJan van Kan
Aug 8, 2002·Kidney International·Hua LiuRadhakrishna Baliga
May 17, 1979·The New England Journal of Medicine·J A BadweyM L Karnovsky
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H M Korchak, G Weissmann
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H M KorchakG Weissmann
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M E Beard, E Holtzman
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T J NealeD Kerjaschki
Oct 16, 2010·The Journal of Biological Chemistry·Hua LiuRadhakrishna Baliga
May 8, 2002·Acta histochemica·W M Frederiks, Helena Vreeling-Sindelárová
Jan 1, 1990·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·A M FiskinR G Garrison
Sep 1, 1996·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·S RozentalW de Souza
Sep 1, 1995·Avian Pathology : Journal of the W.V.P.A·F Gilka, J L Spencer
Apr 12, 2008·Medical Mycology·Maria Fernanda Reis Gavazzoni DiasW de Souza
Jul 1, 1987·The Journal of Cell Biology·J M RobinsonM J Karnovsky
Apr 1, 1978·The Journal of Cell Biology·J M RobinsonM L Karnovsky
Jan 1, 1980·The Journal of Cell Biology·J M ClarkH M Kagan
Nov 26, 2003·The Journal of General Physiology·Gabor L PetheöNicolas Demaurex
Dec 17, 2008·Antioxidants & Redox Signaling·Fredrick D OakleyJohn F Engelhardt
Jul 16, 2008·Molecular Plant-microbe Interactions : MPMI·Nadja SegmüllerPaul Tudzynski
Jun 4, 2010·Plant Physiology·Carla J EatonBarry Scott
Jul 1, 1993·Journal of Microscopy·C J van Noorden, W M Frederiks
Jan 1, 1995·Clinical and Experimental Pharmacology & Physiology. Supplement·H ItoT Suzuki
Sep 1, 1993·The Journal of Eukaryotic Microbiology·N L De Azevedo, W de Souza
Jan 1, 1990·Journal of Clinical Pathology·D E RobertsR Daggett
Feb 1, 1977·The Journal of Clinical Investigation·I M GoldsteinH B Kaplan
Jan 1, 1979·The Journal of Clinical Investigation·B DewaldB M Babior
Apr 1, 1979·The Journal of Clinical Investigation·J T CurnutteM L Karnovsky
Dec 1, 1980·The Journal of Clinical Investigation·Y Suzuki, R I Lehrer
Jan 1, 1981·The Journal of Clinical Investigation·P D Lew, T P Stossel
Jun 1, 1981·The Journal of Clinical Investigation·G L BabiorB M Babior
Aug 1, 1981·The Journal of Clinical Investigation·S K Adeniyi-Jones, M L Karnovsky
Mar 4, 2005·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Miroslava SedláckováJana Záková
Aug 21, 2013·Developmental and Comparative Immunology·Leon GrayferMiodrag Belosevic
Jun 1, 1996·The Histochemical Journal·R J Van den Munckhof
Sep 27, 2013·Fish Physiology and Biochemistry·Marcos Tucunduva de FariaJosé Roberto Machado Cunha da Silva
Jan 1, 1978·The Histochemical Journal·M BorgersW de Cock
Mar 1, 1990·The Histochemical Journal·M BorgersJ Van Reempts
Mar 1, 1990·The Histochemical Journal·R GossrauW M Frederiks
Dec 18, 2013·Antioxidants & Redox Signaling·Shivanjali Joshi-BarrAdah Almutairi
Jan 1, 1991·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·U N RiedeB Seubert
Jan 1, 1989·Critical Reviews in Biochemistry and Molecular Biology·J K Hurst, W C Barrette
Dec 4, 2015·Biology·Jordan W HodgkinsonMiodrag Belosevic
Jan 1, 1994·Progress in Histochemistry and Cytochemistry·K J HalbhuberN Zimmermann
Apr 1, 1992·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·C K LooA W Lykke
Mar 19, 2011·Developmental and Comparative Immunology·Aja M Rieger, Daniel R Barreda
Sep 21, 2007·Research in Veterinary Science·C K MinazakiE Bevilacqua
Jun 10, 2009·Biotechnology Journal·Dorit M W Zander, Matthias Klinger
Mar 10, 2015·Plant Signaling & Behavior·Hidetaka KayaKazuyuki Kuchitsu
Oct 8, 2008·Microscopy Research and Technique·Shaoliang ChenAndrea Polle
May 1, 1987·Journal of Invertebrate Pathology·R DikkeboomW P van der Knaap
Jan 1, 1981·Journal of Ultrastructure Research·U BjörkmanJ F Denef
Mar 1, 1984·Experimental Cell Research·A V PeskinI B Zbarsky
Sep 13, 1995·Biochimica Et Biophysica Acta·J M MayR R Whitesell
Feb 5, 1977·Lancet·D C Taylor
Jun 1, 1979·Cellular Immunology·S E OlabuenagaM M de Bracco
Jul 1, 1983·Cell Biochemistry and Function·N Borgers, A Verheyen
Jan 1, 1982·Annals of the New York Academy of Sciences·J P van DijkanW Harder
May 1, 1983·Journal of Cellular Physiology·J A BadweyM J Karnovsky
Jan 1, 1984·Environmental Mutagenesis·T W Kensler, M A Trush
Aug 1, 1981·Biological Reviews of the Cambridge Philosophical Society·M H Johnson
Feb 1, 1977·The Journal of Pediatrics·L C McPhailC E McCall
Jan 3, 2006·The Journal of Infection·Carolina C de OliveiraDorly de F Buchi
Nov 20, 1998·Archives of Oral Biology·A Oyarzún, P Smith
Feb 1, 1977·Archives of Biochemistry and Biophysics·T J Van Berkel, J K Kruijt
Apr 30, 1981·Biochemical and Biophysical Research Communications·D R Crawford, D L Schneider
Sep 12, 1979·Biochemical and Biophysical Research Communications·J A MillardD L Schneider
Nov 1, 1995·The Histochemical Journal·R J Van den MunckhofW M Frederiks
Jun 5, 1999·Archives of Biochemistry and Biophysics·C AguilarR Vicuña
Aug 22, 2001·Microscopy Research and Technique·C F TeixeiraP E Nagao
Jun 18, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Alessandro ViggianoBruno De Luca
Jun 16, 2009·Molecular Plant-microbe Interactions : MPMI·Jeannine LherminierFrançoise Simon-Plas
Sep 18, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H D Fahimi, E Baumgart
Nov 13, 2015·Biology·Jeffrey J Havixbeck, Daniel R Barreda
Feb 4, 2005·Journal of Leukocyte Biology·Seymour J Klebanoff
May 13, 2015·Infection and Immunity·Lisa G KuhnsRobert J Maier
Mar 3, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Gerald Weissmann
Dec 24, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Andrew StentPhilip Sutton
Apr 7, 1995·The Journal of Biological Chemistry·A R CrossJ T Curnutte
Jan 1, 1976·Annals of the New York Academy of Sciences·L A HarkerJ Glomset
Mar 29, 2003·Physiological Reviews·Thomas E Decoursey
Jan 1, 1982·Annals of the New York Academy of Sciences·R L BaehnerR A Haak
Sep 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Takehiko UeyamaNaoaki Saito
May 15, 1979·Acta Neuropathologica·V SahgalH Singh
Nov 1, 1978·JPEN. Journal of Parenteral and Enteral Nutrition·C JarstrandG Lahnborg
Aug 1, 1989·Clinical Genetics·N CrostiA Serra
Jul 13, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·Y M LeeJ E Repine
Jan 1, 1986·Histochemistry·R T BriggsM J Karnovsky
Jun 13, 2018·Frontiers in Immunology·Leon GrayferMiodrag Belosevic

Related Concepts

Catalase T
Plasma Membrane
Cytoplasmic Granules
Cavia porcellus

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.