PMID: 8585938Nov 1, 1995Paper

Localization of nerves adjacent to goblet cells in rat conjunctiva

Current Eye Research
D A DarttJ D Zieske

Abstract

Neural stimulation of the cornea induces conjunctival goblet cell mucous secretion. Immunofluorescence microscopy was used to determine if nerves are present near conjunctival goblet cells and what types of nerves are present. In euthanized rats, the local anesthetic lidocaine (1%) was placed topically on the ocular surface for 10 min to prevent goblet cell mucous secretion. The ocular surface tissues were removed and either fixed in formaldehyde and then frozen, or frozen first and then post-fixed in formaldehyde. Tissue was sectioned and nerves localized by indirect immunofluorescence microscopy, using antibodies to synaptophysin (indicates nerve, independent of type), vasoactive intestinal peptide (VIP, indicates parasympathetic nerves), tyrosine hydroxylase (TH, indicates sympathetic nerves), dopamine beta-hydroxylase (DBH, indicates sympathetic nerves), phenylethanolamine-N-methyltransferase (PNMT, indicates sympathetic nerves), and calcitonin gene-related peptide (CGRP, indicates sensory nerves). Goblet cells were identified by phase-contrast microscopy. Synpatophysin-containing nerves were present in the basolateral region of conjunctival goblet cells clusters. Nerve fibers immunoreactive to VIP were found in the conjunc...Continue Reading

References

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Jan 1, 1994·Advances in Experimental Medicine and Biology·T L Kessler, D A Dartt

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Citations

Sep 28, 2007·Investigative Ophthalmology & Visual Science·Hiroshi ToshidaAkira Murakami
Jun 24, 2008·Current Eye Research·Ugur KeklikciM Salih Celik
Jun 30, 2015·International Immunopharmacology·Stephanie WiederholdWolfgang Kummer
Mar 13, 2016·Survey of Ophthalmology·William StevensonMingwu Wang
Feb 26, 2004·Veterinary Ophthalmology·Harriet J Davidson, Vanessa J Kuonen
Jun 29, 2004·Progress in Retinal and Eye Research·Michael E Johnson, Paul J Murphy
Jul 24, 2015·Cornea·Stephanie M Cox, Jason J Nichols
Aug 15, 2015·International Ophthalmology·Lorena Romero-Díaz de LeónAlejandro Navas
Jun 22, 1999·Experimental Eye Research·P Seifert, M Spitznas
Aug 1, 1997·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·N A SharifW E Howe
Mar 7, 2003·American Journal of Physiology. Cell Physiology·Harumi KannoDarlene A Dartt
Apr 16, 2015·Vestnik oftalmologii·T N Safonova, L S Pateyuk
Sep 3, 2013·Experimental Eye Research·Ian D Meng, Masayuki Kurose
Oct 6, 2001·Survey of Ophthalmology·C Baudouin
Jan 20, 2004·Experimental Eye Research·Darlene A Dartt

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