PMID: 7536912Apr 1, 1995Paper

Localization of parasite antigens in Cryptosporidium parvum-infected epithelial cells using monoclonal antibodies

Parasitology
V McDonaldF Petry

Abstract

An immunogold ultrastructural study was made of Cryptosporidium parvum-infected intestinal cells from SCID mice to locate parasite antigens recognized by monoclonal antibodies raised against sporozoite or oocyst wall antigens. The results suggested that these antigens were present in more than one life-cycle stage and demonstrated that the intracellular parasite modified the parasitophorous vacuole membrane and villous membrane surrounding the parasite. In an immunofluorescence antibody test monoclonal antibody (MAb) 1B5 reacted with the oocyst wall, MAb 2C3 with the whole sporozoite and MAb 2B2 with the sporozoite surface. Western and dot-blot studies demonstrated that different carbohydrate epitopes were recognized by the respective sporozoite-reactive antibodies. In the ultrastructural examination MAb 1B5 reacted with macro- and microgametocytes as well as the oocyst wall. In the macrogametocyte MAb 1B5 recognized the large electron-dense bodies characteristic of this stage and, in some parasites, the parasitophorous vacuole and the parasite pellicle. The sporozoite-reactive MAbs were able to bind to all developmental stages. These antibodies recognized the parasite cytoplasm and, additionally, MAb 2B2 produced substantial l...Continue Reading

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Citations

Nov 27, 1999·International Journal for Parasitology·D C de GraafA Bonnin
Jun 13, 2003·Micron : the International Research and Review Journal for Microscopy·J Robin HarrisFranz Petry
Nov 5, 2004·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Franz Petry
Oct 17, 2013·Parasitology·Janka MelicherováAndrea Valigurová
Sep 9, 2016·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Marwa M I GhallabAyman A El-Badry
Jan 17, 2004·Parasitology Research·Steven P O'HaraJim Jung-Ching Lin
Sep 6, 2011·International Journal for Parasitology·P Karanis, H M Aldeyarbi
Apr 5, 2011·Microbes and Infection·Steven P O'Hara, Xian-Ming Chen
May 3, 2008·Molecular Microbiology·Victoria CarterJohannes T Dessens
Jun 19, 2013·Molecular Microbiology·Annie Z TrempJohannes T Dessens
Nov 24, 2012·Parasite Immunology·V McDonaldF Petry
Apr 23, 2015·International Journal for Parasitology·Sadia SaeedJohannes T Dessens
Sep 20, 2008·Trends in Parasitology·Hanna BorowskiR C Andrew Thompson
Oct 18, 2011·Experimental Parasitology·Migyo JoungJae-Ran Yu

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