Localization of peptide transporter in nuclei and lysosomes of the pancreas

International Journal of Pancreatology : Official Journal of the International Association of Pancreatology
D E BockmanF H Leibach

Abstract

These studies show for the first time the localization of a H+/peptide cotrasporter in nuclei of vascular smooth muscle cells and Schwann cells and its localization in lysosomes of the exocrine pancreas. It is likely that the transporter functions to move small peptides from the lysosome to the cytoplasm following intralysosomal protein degradation. The nature of the transporter function in the nucleus remains to be determined, including the possibility that peptide signaling molecules may be transmitted between nucleus and cytoplasm. PEPT1 transports di- and tripeptides through plasma membranes. Peptides are cotransported with H+, thus deriving the energy for the active transport process from an electrochemical H+ gradient. The main regions in which PEPT1 has been thought to function are the plasma membranes of the small intestinal epithelial cells for absorption of protein digestion products and in the kidney tubules for recovery of small peptides from the glomerular filtrate. Pancreas was removed from rats and quick frozen with liquid nitrogen. Frozen sections were fixed in cold acetone. Sections were incubated with primary antibody against PEPT1, followed by a secondary antibody conjugated with fluorescein, then examined wi...Continue Reading

References

Aug 1, 1991·Current Opinion in Cell Biology·V Ganapathy, F H Leibach
May 24, 1990·Biochimica Et Biophysica Acta·S J Bird, J B Lloyd
Jan 1, 1984·Contributions to Nephrology·V GanapathyF H Leibach
Mar 27, 1996·Biochemical and Biophysical Research Communications·H OgiharaK Takata
Jan 1, 1996·Annual Review of Nutrition·F H Leibach, V Ganapathy
Jul 1, 1996·Pharmaceutical Research·A Tsuji, I Tamai

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Citations

May 5, 2001·The Biochemical Journal·K SakataM Tohyama
Jan 13, 2005·British Journal of Pharmacology·David J KennedyDavid T Thwaites
Jan 28, 2010·Pharmacological Reviews·Curtis D Klaassen, Lauren M Aleksunes
Sep 2, 2011·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Dilara JapparDavid E Smith
Jan 1, 2016·BioImpacts : BI·Jaleh BararYadollah Omidi
Apr 3, 2019·Biochemical Society Transactions·Kiran Javed, Stephen J Fairweather
Mar 14, 2008·Alcoholism, Clinical and Experimental Research·Kaixun LiDaryl L Davies
Aug 1, 2008·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·I Rubio-Aliaga, H Daniel
Jun 9, 2006·Journal of Medicinal Chemistry·Balvinder S VigTeresa N Faria
Feb 13, 2001·The American Journal of Pathology·D A GronebergA Fischer

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