Localization of phospholipids in human catecholamine neurons with Baker's acid haematein.

Journal of Neurocytology
M T PanayotacopoulouMarietta R Issidorides

Abstract

Protein bodies in catecholamine neurons of the normal human brain contain arginine-rich proteins similar to those present in the core of Lewy bodies in Parkinsonian brains. Lewy bodies are known also to contain phospholipids in their core, demonstrable with Baker's acid haematein. We used Baker's procedure on catecholamine neurons of normal brains to test whether the protein bodies also contain phospholipids. Postmortem tissues of three control individuals were used in this study. Locus coeruleus and substantia nigra were dissected out from the fresh brains and two sets of blocks were made from each area. One set was fixed in formol-calcium for the preservation of lipids, the second was fixed in Bouin's solution and treated with hot pyridine for the extraction of lipids. Finally, both sets were subjected to the same chroming and staining procedure according to Baker. In catecholamine neurons the protein bodies were stained with acid haematein, indicating the presence of phospholipids. Since this staining resisted pyridine extraction we conclude that these phospholipids are firmly bound to the basic proteins. Thus, protein bodies in healthy catecholamine neurons give the same positive reaction for phospholipids as that reported ...Continue Reading

References

Apr 26, 1976·Acta Neuropathologica·E Ohama, F Ikuta
May 24, 1978·Acta Neuropathologica·K Kosaka
Nov 1, 1988·Journal of Neuropathology and Experimental Neurology·P G GallowayG Perry
Jan 1, 1969·Advances in Lipid Research·C W Adams
Dec 1, 1969·Archives of Neurology·W A den Jager
Oct 1, 1982·Archives of Neurology·M T Panayotacopoulou, M R Issidorides
Nov 1, 1984·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M T Panayotacopoulou, M R Issidorides

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Citations

Jan 1, 1991·Journal of Neural Transmission. Parkinson's Disease and Dementia Section·M R IssidoridesM D Yahr

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