PMID: 6970049Oct 28, 1980Paper

Localization of photosynthetic membrane components in Rhodopseudomonas sphaeroides by a radioactive labeling procedure

Biochemistry
G A Francis, W R Richards

Abstract

Reduction with [3H]KBH4 of Schiff's bases generated by reaction with pyridoxal 5'-phosphate (which cannot penetrate the intact cytoplasmic membrane) yields tritium-labeled derivatives of both proteins and lipids accessible on the periplasmic side of the cytoplasmic membrane. Application of this technique to phototrophically grown Rhodopseudomonas sphaeroides labeled both the cell envelope and chromatophore fractions. The technique was also applied to R. sphaeroides harvested at various times during an adaptation from heterotrophic to phototrophic growth conditions. The specific activity of the chromatophore fraction after 20 h of adaptation was 76% of that found at the beginning, indicating that the intracytoplasmic membranes and cytoplasmic membrane form a continuous membrane system, with the majority of the intracytoplasmic membranes accessible to the external medium throughout the adaptation. The identity of the proteins labeled by this technique was investigated in two fractions labeled after cell disruption: normal "inside-out" chromatophores and "right-side-out" membrane vesicles isolated by lysozyme--osmotic shock treatment of cells grown in high light intensity (15000 lx). The results after sodium dodecyl sulfate--polya...Continue Reading

References

Oct 31, 1975·Biochimica Et Biophysica Acta·V Braun
Oct 1, 1975·European Journal of Biochemistry·A HochmanC Carmeli
Dec 14, 1972·Biochimica Et Biophysica Acta·R K Clayton, B J Clayton
Dec 23, 1967·Nature·D Bownds
Oct 1, 1972·Archives of Biochemistry and Biophysics·K D GibsonR A Niederman
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Feb 1, 1972·Photochemistry and Photobiology·J Aagaard, W R Sistrom
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R M BroglieR K Clayton
Feb 1, 1963·The Journal of Cell Biology·G COHEN-BAZIRE, R KUNISAWA

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Citations

Apr 27, 1999·Biochimica Et Biophysica Acta·T KawaneT Ishii
Dec 15, 1983·European Journal of Biochemistry·K PalczewskiM Kochman
Jul 27, 2010·Biophysical Journal·Melih SenerKlaus Schulten

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