Localization of plasminogen activator and inhibitor, LH and androgen receptors and inhibin subunits in monkey epididymis

Molecular Human Reproduction
T ZhangY X Liu

Abstract

Epididymis is a site of sperm maturation and storage. Limited and directed-proteolysis regulated by plasminogen activator (PA), plasminogen activator inhibitor type-1 (PAI-1) and other related factors may play an essential role in these processes. Our previous studies have demonstrated that rat epididymis expressed luteinizing hormone receptor (LHR), tissue type (t) and urokinase type (u)PA, mRNAs, and tPA activity was stimulated in vitro by human chorionic gonoadotrophin (HCG). In the present study we further examined localization of mRNAs for tPA, uPA, LHR, androgen receptor (AR), as well as inhibin subunits alpha, betaA and betaB in rhesus monkey epididymis. Using in-situ hybridization with digoxygenin-labelled cRNA probes, we have demonstrated that tPA and PAI-1 mRNAs were localized in epithelial cells of adult monkey epididymis. uPA mRNA was localized in the same areas, but to a much smaller extent. tPA, uPA and PAI-1 mRNAs were greatly expressed in the caput and corpus of adult epididymis than in other regions. In-vitro experiments showed that both tPA and uPA activities in epididymal cells were dramatically stimulated by HCG, but not by follicle stimulating hormone (FSH). LHR (but not FSH receptor) and AR mRNAs were loca...Continue Reading

Citations

May 4, 2002·Molecular and Cellular Endocrinology·C V Rao, Z M Lei
Jan 26, 2011·Reproductive Toxicology·Daniela A Fossato da SilvaWilma De G Kempinas
Feb 6, 2008·Fertility and Sterility·Inge M W EbischRégine P M Steegers-Theunissen
Feb 6, 2008·Molecular and Cellular Endocrinology·Tamar D Schirman-HildesheimYitzhak Koch
Jan 13, 2015·Asian Journal of Andrology·Ying QinLing Zhang
Nov 30, 2007·Theriogenology·M P TsantarliotouA Smokovitis
Mar 25, 2018·Journal of Assisted Reproduction and Genetics·Juan Carlos Martinez-SotoJoaquín Gadea
Jun 10, 2018·Reproduction in Domestic Animals = Zuchthygiene·Florencia BeracocheaRodolfo Ungerfeld
Mar 2, 2010·Reproduction, Fertility, and Development·Manuel NistalMaria P De Miguel

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