PMID: 9170149Apr 1, 1997Paper

Localization of polymorphic N-acetyltransferase (NAT2) in tissues of inbred mice

Pharmacogenetics
L A StanleyE Sim

Abstract

Like humans, mice exhibit polymorphism in the N-acetylation of aromatic amines, many of which are toxic and/or carcinogenic. Mice have three N-acetyltransferase (Nat) genes, Nat1, Nat2 and Nat3, and Nat2 is known to be polymorphic. There is a dramatic difference in the acetylation of NAT2 substrates by blood from fast (C57BL/6J) compared with slow acetylator (A/J) mice. However, the acetylation of these substrates by liver cytosols from the two strains is very similar. In order to determine whether the expression of the NAT2 protein corresponded with the activities measured, a polyclonal antipeptide antisera was raised against the C-terminal decapeptide of NAT2 and characterized using recombinant murine NAT2 antigen. Enzyme-linked immunosorbent assays (ELISAs) demonstrated that the anti-NAT2 antiserum bound in a concentration-dependent fashion to recombinant NAT2. Immunochemical analysis of mouse liver cytosols from C57BL/6J or A/J livers indicated that the level of NAT2 protein expressed in the two strains was similar. Immunohistochemical staining of C57BL/6J liver with anti-NAT2 antiserum showed that NAT2 was expressed in hepatocytes throughout the liver although the intensity of staining in the perivenous (centrilobular) reg...Continue Reading

Citations

Sep 19, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·David W HeinMark A Doll
Jul 22, 2006·The Journal of Pharmacology and Experimental Therapeutics·Jennifer A LoehleDavid W Hein
Mar 29, 2014·Drug Metabolism Reviews·Ursula Gundert-RemyPeter H Roos
Jul 22, 2008·Drug Metabolism Reviews·Edith SimSotiria Boukouvala
Nov 1, 2005·Drug Metabolism Reviews·Sotiria Boukouvala, Giannoulis Fakis
Sep 27, 2007·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Larissa WakefieldEdith Sim
Sep 25, 2008·The Journal of Pharmacy and Pharmacology·K Sandy PangHuadong Sun
Aug 7, 2003·Chemico-biological Interactions·Charlene A McQueenMartin A Philbert
Oct 27, 2007·Biochemical and Biophysical Research Communications·Larissa WakefieldEdith Sim
May 6, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Larissa WakefieldEdith Sim
Apr 20, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Verónica Ramírez-Alcántara, Marshall H Montrose

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