PMID: 3755813Aug 15, 1986Paper

Localization of prodynorphin messenger rna in rat brain by in situ hybridization using a synthetic oligonucleotide probe

Neuroscience Letters
B J MorrisA Herz

Abstract

An in situ hybridization technique has been developed to study the distribution of messenger RNA (mRNA) encoding prodynorphin (proenkephalin B) in sections of rat brain. A 100-mer oligonucleotide was used as a template to synthesize a 32P-labelled DNA probe complementary to the coding region of rat prodynorphin mRNA. Using this probe, dense labelling was observed in the supraoptic and paraventricular nuclei of the hypothalamus, in the striatum and in the dentate gyrus. The results show for the first time the localization of prodynorphin mRNA in rat brain, and additionally demonstrate the usefulness of oligonucleotides to detect rare mRNAs by in situ hybridization.

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Citations

Jul 10, 2013·Psychopharmacology·Ashlee Van't Veer, William A Carlezon
Dec 1, 1987·Pharmacology, Biochemistry, and Behavior·J H Van DaalJ H Van Abeelen
Sep 17, 1993·Brain Research. Developmental Brain Research·F M Laurent-HuckJ M Felix
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Dec 3, 1999·Progress in Neuro-psychopharmacology & Biological Psychiatry·M S Cowen, A J Lawrence
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Sep 1, 1995·The European Journal of Neuroscience·P RomualdiM Simonato
Jan 1, 1988·Peptides·J A StiversJ N Crawley
May 20, 2015·Acta Pharmacologica Sinica·Ai HangJing-gen Liu
Jul 1, 1993·Trends in Endocrinology and Metabolism : TEM·A N Margioris
Feb 22, 2018·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·E DomiM Heilig
Mar 10, 2001·The Journal of Comparative Neurology·C F EliasJ K Elmquist

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