PMID: 7536427Apr 1, 1995Paper

Localization of regions affecting an allosteric transition in cyclic nucleotide-activated channels

Neuron
S E Gordon, W N Zagotta

Abstract

Sensory transduction in olfactory receptors and photoreceptors is mediated by cyclic nucleotide-activated ion channels. We have studied the gating mechanism in olfactory and rod channels expressed in Xenopus oocytes. We report that the differences in cyclic nucleotide affinity and efficacy between these channels resulted from sequence differences outside the cyclic nucleotide-binding domain, especially in the amino-terminal domain, influencing the free energy of the closed to open allosteric conformational change. In addition, Ni2+ inhibited activation of the olfactory channel, decreasing both the maximum current and the apparent affinity for cyclic nucleotides. Ni2+ exerted its effect by binding preferentially to the closed configuration of the channel, thereby destabilizing the opening conformational change. We have localized this inhibition to a single histidine (H396) following the last transmembrane segment, suggesting a role for this region in channel gating.

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Citations

May 1, 1996·Journal of Neurobiology·M C Broillet, S Firestein
Jun 1, 1996·Journal of Bioenergetics and Biomembranes·W N Zagotta
Aug 1, 1995·Current Opinion in Neurobiology·U B Kaupp
Jun 1, 1995·Current Opinion in Neurobiology·A L Zimmerman
Aug 1, 1996·Current Opinion in Neurobiology·R S Molday
Nov 1, 1996·Trends in Cardiovascular Medicine·M BielF Hofmann
Mar 25, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sven SchünkeDieter Willbold
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·S E Gordon, W N Zagotta
Jan 13, 2011·The Journal of Biological Chemistry·Carmen A Ufret-VincentySharona E Gordon
Mar 28, 2008·The Journal of Biological Chemistry·Kimberley B CravenWilliam N Zagotta
Jan 1, 1997·The Journal of General Physiology·A A FodorW N Zagotta
Mar 1, 1997·The Journal of General Physiology·C Y Tang, D M Papazian
Aug 1, 1997·The Journal of General Physiology·S ShyngC G Nichols
Nov 14, 1997·The Journal of General Physiology·A A FodorW N Zagotta
Sep 3, 1998·The Journal of General Physiology·S TrappF M Ashcroft
Jan 5, 1999·The Journal of General Physiology·P PaolettiS A Siegelbaum
May 6, 1999·The Journal of General Physiology·E R Sunderman, W N Zagotta
Aug 5, 2000·The Journal of General Physiology·S E Gordon
Aug 5, 2000·The Journal of General Physiology·T R MiddendorfD A Baylor
Aug 5, 2000·The Journal of General Physiology·T R Middendorf, R W Aldrich
Dec 1, 2000·The Journal of General Physiology·J I CraryA L Zimmerman
Feb 27, 2001·The Journal of General Physiology·E Molokanova, R H Kramer
Aug 2, 2001·The Journal of General Physiology·H MöttigK Benndorf
Feb 26, 2004·The Journal of General Physiology·Tamara RosenbaumSharona E Gordon
Sep 1, 2004·The Journal of General Physiology·Crina M NimigeanChristopher Miller
Dec 2, 2004·The Journal of General Physiology·Kimberley B Craven, William N Zagotta
Mar 2, 2005·The Journal of General Physiology·Li Hua, Sharona E Gordon
Aug 31, 2006·The Journal of General Physiology·Leon D Islas, William N Zagotta
Jul 29, 2009·The Journal of General Physiology·Juan Ramón Martínez-FrançoisZhe Lu
Mar 27, 2013·The Journal of General Physiology·Gucan DaiMichael D Varnum
Jun 17, 1998·The EMBO Journal·S J TuckerF M Ashcroft
Jul 22, 1999·Annals of the New York Academy of Sciences·M C Broillet, S Firestein
Jul 22, 1999·Annals of the New York Academy of Sciences·B Santoro, G R Tibbs
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Kimberly Matulef, William N Zagotta
Feb 8, 2006·Annual Review of Physiology·Kimberley B Craven, William N Zagotta
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·M E GrunwaldK W Yau
May 30, 2002·Proceedings of the National Academy of Sciences of the United States of America·Dylan M DeanAnita L Zimmerman

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