Localization of Saccharomyces cerevisiae protein phosphatase 2A subunits throughout mitotic cell cycle

doi:10.1091/mbc.02-05-0065

PMID: 12388751DOI: 10.1091/mbc.02-05-0065Oct 22, 2002Paper

Localization of Saccharomyces cerevisiae protein phosphatase 2A subunits throughout mitotic cell cycle

Molecular Biology of the Cell

Matthew S Gentry, Richard L Hallberg

Get PDF

Abstract

Protein phosphatase 2A (PP2A) regulates a broad spectrum of cellular processes. This enzyme is a collection of varied heterotrimeric complexes, each composed of a catalytic (C) and regulatory (B) subunit bound together by a structural (A) subunit. To understand the cell cycle dynamics of this enzyme population, we carried out quantitative and qualitative analyses of the PP2A subunits of Saccharomyces cerevisiae. We found the following: the level of each subunit remained constant throughout the cell cycle; there is at least 10 times more of one of the regulatory subunits (Rts1p) than the other (Cdc55p); Tpd3p, the structural subunit, is limiting for both catalytic and regulatory subunit binding. Using green fluorescent protein-tagged forms of each subunit, we monitored the sites of significant accumulation of each protein throughout the cell cycle. The two regulatory subunits displayed distinctly different dynamic localization patterns that overlap with the A and C subunits at the bud tip, kinetochore, bud neck, and nucleus. Using strains null for single subunit genes, we confirmed the hypothesis that regulatory subunits determine sites of PP2A accumulation. Although Rts1p and Tpd3p required heterotrimer formation to achieve nor...Continue Reading

References

Regulation of protein serine-threonine phosphatase type-2A by tyrosine phosphorylation.

Aug 28, 1992·Science·J ChenD L Brautigan

Identification of binding sites on the regulatory A subunit of protein phosphatase 2A for the catalytic C subunit and for tumor antigens of simian virus 40 and polyomavirus.

Nov 1, 1992·Molecular and Cellular Biology·R RuedigerG Walter

Inactivation of the protein phosphatase 2A regulatory subunit A results in morphological and transcriptional defects in Saccharomyces cerevisiae.

Nov 1, 1992·Molecular and Cellular Biology·W van ZylJ R Broach

Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly

Jul 1, 1992·The Journal of Cell Biology·M A HoytW S Saunders

Protein phosphatase 2A in Saccharomyces cerevisiae: effects on cell growth and bud morphogenesis.

Oct 1, 1991·Molecular and Cellular Biology·H RonneJ O Nehlin

CDC55, a Saccharomyces cerevisiae gene involved in cellular morphogenesis: identification, characterization, and homology to the B subunit of mammalian type 2A protein phosphatase.

Nov 1, 1991·Molecular and Cellular Biology·A M HealyJ R Pringle

A new vital stain for visualizing vacuolar membrane dynamics and endocytosis in yeast

Mar 1, 1995·The Journal of Cell Biology·T A Vida, S D Emr

SCS1, a multicopy suppressor of hsp60-ts mutant alleles, does not encode a mitochondrially targeted protein.

Oct 1, 1995·Molecular and Cellular Biology·Y Shu, R L Hallberg

A novel pool of protein phosphatase 2A is associated with microtubules and is regulated during the cell cycle

Mar 1, 1995·The Journal of Cell Biology·E SontagM C Mumby

Protein serine/threonine phosphatases: structure, regulation, and functions in cell growth

Oct 1, 1993·Physiological Reviews·M C Mumby, G Walter

NDC10: a gene involved in chromosome segregation in Saccharomyces cerevisiae

May 1, 1993·The Journal of Cell Biology·P Y Goh, J V Kilmartin

Spc42p: a phosphorylated component of the S. cerevisiae spindle pole body (SPD) with an essential function during SPB duplication

Mar 1, 1996·The Journal of Cell Biology·A D Donaldson, J V Kilmartin

The B56 family of protein phosphatase 2A (PP2A) regulatory subunits encodes differentiation-induced phosphoproteins that target PP2A to both nucleus and cytoplasm.

Sep 6, 1996·The Journal of Biological Chemistry·B McCrightD M Virshup

Nutrients, via the Tor proteins, stimulate the association of Tap42 with type 2A phosphatases

Aug 1, 1996·Genes & Development·C J Di Como, K T Arndt

Protein phosphatase 2A regulates MPF activity and sister chromatid cohesion in budding yeast

Dec 1, 1996·Current Biology : CB·J MinshullA W Murray

Cdc55p, the B-type regulatory subunit of protein phosphatase 2A, has multiple functions in mitosis and is required for the kinetochore/spindle checkpoint in Saccharomyces cerevisiae.

Feb 1, 1997·Molecular and Cellular Biology·Y Wang, D J Burke

Separation of PP2A core enzyme and holoenzyme with monoclonal antibodies against the regulatory A subunit: abundant expression of both forms in cells.

Mar 1, 1997·Molecular and Cellular Biology·E KremmerG Walter

Molecular genetic analysis of Rts1p, a B' regulatory subunit of Saccharomyces cerevisiae protein phosphatase 2A.

Jun 1, 1997·Molecular and Cellular Biology·Y ShuR Hallberg

Yeast nuclei display prominent centromere clustering that is reduced in nondividing cells and in meiotic prophase

May 16, 1998·The Journal of Cell Biology·Q JinJ Loidl

Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization

Dec 8, 1998·Molecular Biology of the Cell·P T SpellmanB Futcher

Role of PP2A in intracellular signal transduction pathways.

Dec 16, 1998·Frontiers in Bioscience : a Journal and Virtual Library·A H Schönthal

Regulation of protein kinase cascades by protein phosphatase 2A

May 14, 1999·Trends in Biochemical Sciences·T A MillwardB A Hemmings

Tor proteins and protein phosphatase 2A reciprocally regulate Tap42 in controlling cell growth in yeast

May 18, 1999·The EMBO Journal·Y Jiang, J R Broach

Immunological analysis of yeast chromatin

Jun 18, 1999·Methods in Enzymology·P B Meluh, J R Broach

The morphogenesis checkpoint in Saccharomyces cerevisiae: cell cycle control of Swe1p degradation by Hsl1p and Hsl7p.

Sep 22, 1999·Molecular and Cellular Biology·J N McMillanD J Lew

Hsl7 localizes to a septin ring and serves as an adapter in a regulatory pathway that relieves tyrosine phosphorylation of Cdc28 protein kinase in Saccharomyces cerevisiae.

Sep 22, 1999·Molecular and Cellular Biology·M J ShulewitzJ Thorner

Protein phosphatase 2A: a panoply of enzymes

Mar 14, 2000·Current Opinion in Cell Biology·D M Virshup

Protein phosphatase 2A: a definite player in viral and parasitic regulation

May 19, 2000·Microbes and Infection·A GarciaE Sontag

The B56alpha regulatory subunit of protein phosphatase 2A is a target for regulation by double-stranded RNA-dependent protein kinase PKR.

Jun 24, 2000·Molecular and Cellular Biology·Z Xu, B R Williams

Transient sister chromatid separation and elastic deformation of chromosomes during mitosis in budding yeast

Jul 13, 2000·Cell·X HeP K Sorger

Loss of a protein phosphatase 2A regulatory subunit (Cdc55p) elicits improper regulation of Swe1p degradation.

Oct 12, 2000·Molecular and Cellular Biology·H YangR L Hallberg

Carboxymethylation of the PP2A catalytic subunit in Saccharomyces cerevisiae is required for efficient interaction with the B-type subunits Cdc55p and Rts1p

Oct 20, 2000·The Journal of Biological Chemistry·H WeiD C Pallas

Carboxyl methylation of the phosphoprotein phosphatase 2A catalytic subunit promotes its functional association with regulatory subunits in vivo

Nov 4, 2000·The EMBO Journal·J WuJ R Broach

Mutation of the C-terminal leucine residue of PP2Ac inhibits PR55/B subunit binding and confers supersensitivity to microtubule destabilization in Saccharomyces cerevisiae

Dec 29, 2000·Molecular & General Genetics : MGG·D R Evans, B A Hemmings

Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling

Feb 15, 2001·The Biochemical Journal·V Janssens, J Goris

Budding yeast chromosome structure and dynamics during mitosis

Mar 21, 2001·The Journal of Cell Biology·C G PearsonK Bloom

A mechanism for nuclear positioning in fission yeast based on microtubule pushing

Apr 20, 2001·The Journal of Cell Biology·P T TranF Chang

Molecular analysis of kinetochore-microtubule attachment in budding yeast

Aug 21, 2001·Cell·X HeP K Sorger

The protein phosphatase 2A B'-regulatory subunit par1p is implicated in regulation of the S. pombe septation initiation network

Nov 15, 2001·FEBS Letters·X Le GoffV Simanis

Actions of PP2A on the MAP kinase pathway and apoptosis are mediated by distinct regulatory subunits

Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Adam M SilversteinMarc C Mumby

❮ Previous

Next ❯

Citations

Emerging roles of nuclear protein phosphatases

Feb 24, 2007·Nature Reviews. Molecular Cell Biology·Greg B G MoorheadAnnegret Ulke-Lemée

The B56gamma3 regulatory subunit of protein phosphatase 2A (PP2A) regulates S phase-specific nuclear accumulation of PP2A and the G1 to S transition.

May 8, 2010·The Journal of Biological Chemistry·Ting-Yuan LeeChi-Wu Chiang

Stress-induced ceramide-activated protein phosphatase can compensate for loss of amphiphysin-like activity in Saccharomyces cerevisiae and functions to reinitiate endocytosis.

Mar 4, 2009·The Journal of Biological Chemistry·Paula C McCourtJoseph T Nickels

The PXL1 gene of Saccharomyces cerevisiae encodes a paxillin-like protein functioning in polarized cell growth

Feb 10, 2004·Molecular Biology of the Cell·Nancy A MackinScott E Erdman

The role of Cdc55 in the spindle checkpoint is through regulation of mitotic exit in Saccharomyces cerevisiae

Nov 30, 2005·Molecular Biology of the Cell·Christopher M Yellman, Daniel J Burke

The Zds proteins control entry into mitosis and target protein phosphatase 2A to the Cdc25 phosphatase

Dec 2, 2010·Molecular Biology of the Cell·Sidonie WickyDouglas R Kellogg

Phosphorylation of the Ndc80 complex protein, HEC1, by Nek2 kinase modulates chromosome alignment and signaling of the spindle assembly checkpoint.

Aug 13, 2011·Molecular Biology of the Cell·Randy WeiWen-Hwa Lee

A phosphatase threshold sets the level of Cdk1 activity in early mitosis in budding yeast.

Aug 19, 2011·Molecular Biology of the Cell·Stacy L HarveyDouglas R Kellogg

The protein phosphatase 2A functions in the spindle position checkpoint by regulating the checkpoint kinase Kin4.

Jul 17, 2009·Genes & Development·Leon Y Chan, Angelika Amon

Disparate roles for the regulatory A subunit isoforms in Arabidopsis protein phosphatase 2A

Feb 20, 2004·The Plant Cell·Hong-Wei ZhouAlison DeLong

Genetic interactions among regulators of septin organization

Aug 11, 2004·Eukaryotic Cell·Amy S GladfelterDaniel J Lew

PP2A phosphatase activity is required for stress and Tor kinase regulation of yeast stress response factor Msn2p

Oct 8, 2004·Eukaryotic Cell·Arti SanthanamStephen Garrett

A novel assay for protein phosphatase 2A (PP2A) complexes in vivo reveals differential effects of covalent modifications on different Saccharomyces cerevisiae PP2A heterotrimers

Jun 11, 2005·Eukaryotic Cell·Matthew S GentryDavid C Pallas

Adenovirus protein E4orf4 induces premature APCCdc20 activation in Saccharomyces cerevisiae by a protein phosphatase 2A-dependent mechanism.

Feb 19, 2010·Journal of Virology·Melissa Z MuiPhilip E Branton

Yeast protein phosphatase 2A-Cdc55 regulates the transcriptional response to hyperosmolarity stress by regulating Msn2 and Msn4 chromatin recruitment.

Jan 1, 2013·Molecular and Cellular Biology·Wolfgang ReiterGustav Ammerer

Regulation of the cell cycle by protein phosphatase 2A in Saccharomyces cerevisiae

Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Yu Jiang

Septins: molecular partitioning and the generation of cellular asymmetry.

Aug 28, 2009·Cell Division·Michael A McMurray, Jeremy Thorner

Monitoring spindle orientation: Spindle position checkpoint in charge

Dec 15, 2010·Cell Division·Ayse K CaydasiGislene Pereira

Proteins in the nutrient-sensing and DNA damage checkpoint pathways cooperate to restrain mitotic progression following DNA damage

Jul 23, 2011·PLoS Genetics·Jennifer S SearleYolanda Sanchez

Phosphorylation provides a negative mode of regulation for the yeast Rab GTPase Sec4p.

Sep 21, 2011·PloS One·Christopher D HegerRuth N Collins

Centromere binding and a conserved role in chromosome stability for SUMO-dependent ubiquitin ligases

Jun 21, 2013·PloS One·Loes A L van de PaschFrank C P Holstege

Cloning and characterization of TaPP2AbB"-α, a member of the PP2A regulatory subunit in wheat

Apr 9, 2014·PloS One·Dan LiuRuilian Jing

Identification of the PP2A-interacting region of heat shock transcription factor 2

Aug 11, 2007·Cell Stress & Chaperones·Hongyan XingKevin D Sarge

A novel septin-associated protein, Syp1p, is required for normal cell cycle-dependent septin cytoskeleton dynamics in yeast.

Sep 16, 2008·Genetics·Wenjie QiuMingjie Cai

Sgo1 regulates both condensin and Ipl1/Aurora B to promote chromosome biorientation

Jun 20, 2014·PLoS Genetics·Karolina PeplowskaZuzana Storchova

Regulation of pol III transcription by nutrient and stress signaling pathways

Nov 21, 2012·Biochimica Et Biophysica Acta·Robyn D Moir, Ian M Willis

A link between mitotic entry and membrane growth suggests a novel model for cell size control.

Mar 28, 2012·The Journal of Cell Biology·Steph D AnastasiaDouglas R Kellogg

Spatial regulation of Cdc55-PP2A by Zds1/Zds2 controls mitotic entry and mitotic exit in budding yeast.

May 4, 2011·The Journal of Cell Biology·Valentina Rossio, Satoshi Yoshida

Zds1/Zds2-PP2ACdc55 complex specifies signaling output from Rho1 GTPase.

Jan 6, 2016·The Journal of Cell Biology·Erin M JonassonSatoshi Yoshida

Functions of B56-containing PP2As in major developmental and cancer signaling pathways

Oct 12, 2010·Life Sciences·Jing Yang, Christopher Phiel

Systems biology of the cell cycle of Saccharomyces cerevisiae: From network mining to system-level properties

May 26, 2009·Biotechnology Advances·Lilia AlberghinaIvan Orlandi

PP2A holoenzyme assembly: in cauda venenum (the sting is in the tail)

Feb 23, 2008·Trends in Biochemical Sciences·Veerle JanssensJozef Goris

Shared components of protein complexes--versatile building blocks or biochemical artefacts?

Nov 20, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Roland KrauseThomas Dandekar

The alpha4-containing form of protein phosphatase 2A in liver and hepatic cells.

Jun 11, 2008·Journal of Cellular Biochemistry·Sunny J-S YooPhilip A Gruppuso

The biogenesis of active protein phosphatase 2A holoenzymes: a tightly regulated process creating phosphatase specificity.

Mar 27, 2012·The FEBS Journal·Ward SentsVeerle Janssens

Protein phosphatase 2A regulatory subunits perform distinct functional roles in the maize pathogen Fusarium verticillioides

Mar 5, 2013·Molecular Plant Pathology·Joon-Hee ShinWon-Bo Shim

Proteome-wide search for PP2A substrates in fission yeast

Mar 19, 2014·Proteomics·Manuel BernalRafael R Daga

All roads lead to PP2A: exploiting the therapeutic potential of this phosphatase

Oct 29, 2015·The FEBS Journal·Jaya SangodkarGoutham Narla

Cyclin G2 is a centrosome-associated nucleocytoplasmic shuttling protein that influences microtubule stability and induces a p53-dependent cell cycle arrest

Nov 25, 2006·Experimental Cell Research·Aruni S Arachchige DonMary C Horne

Tau hyperphosphorylation correlates with reduced methylation of protein phosphatase 2A

Jul 1, 2008·Neurobiology of Disease·Xin-Wen ZhouJin-Jing Pei

Comparative genetic analysis of PP2A-Cdc55 regulators in budding yeast

May 8, 2014·Cell Cycle·Valentina RossioSatoshi Yoshida

Phosphorylation-dependent regulation of septin dynamics during the cell cycle

Mar 15, 2003·Developmental Cell·Jeroen DobbelaereYves Barral

Protein phosphatase 2A plays a crucial role in Giardia lamblia differentiation

Jan 6, 2007·Molecular and Biochemical Parasitology·Tineke LauwaetFrances D Gillin

Downregulation of PP2A(Cdc55) phosphatase by separase initiates mitotic exit in budding yeast

May 23, 2006·Cell·Ethel QueraltFrank Uhlmann

Protein phosphatase 2A is involved in the tyrosine hydroxylase phosphorylation regulated by α-synuclein

Jan 9, 2015·Neurochemical Research·Gao HuaYang Hui

An integrated overview of spatiotemporal organization and regulation in mitosis in terms of the proteins in the functional supercomplexes

Nov 18, 2014·Frontiers in Microbiology·Yueyuan ZhengJian Ren

Visualization of subunit interactions and ternary complexes of protein phosphatase 2A in mammalian cells

Dec 24, 2014·PloS One·Shu-Ting MoChi-Wu Chiang

Tpd3-Pph21 phosphatase plays a direct role in Sep7 dephosphorylation in Candida albicans

Mar 19, 2016·Molecular Microbiology·Qizheng LiuYue Wang

A simple enrichment procedure improves detection of membrane proteins by immunoblotting

Nov 15, 2016·BioTechniques·Azamat Karginov, Michael Agaphonov

The scaffolding A/Tpd3 subunit and high phosphatase activity are dispensable for Cdc55 function in the Saccharomyces cerevisiae spindle checkpoint and in cytokinesis.

Sep 7, 2004·The Journal of Biological Chemistry·Roni KorenTamar Kleinberger

❮ Previous

Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.