Localization of soluble guanylyl cyclase in the superficial dorsal horn

The Journal of Comparative Neurology
Jin-Dong Ding, Richard J Weinberg

Abstract

Nitric oxide (NO) has been implicated in pain processing at the spinal level, but the mechanisms mediating its effects remain unclear. In the present work, we studied the organization of the major downstream effector of NO, soluble guanylyl cyclase (sGC), in the superficial dorsal horn of rat. Almost all neurokinin 1 (NK1) receptor-positive neurons in lamina I (a major source of ascending projections) were strongly immunopositive for sGC. Many local circuit neurons in laminae I-II also stained for sGC, but less intensely. Numerous fibers, presumably of unmyelinated primary afferent (C fiber) origin, stained for calcitonin gene-related peptide or isolectin B4, but none of these was immunopositive for sGC. These data, along with immunoelectron microscopy results, imply that unmyelinated primary afferent fibers terminating in the superficial dorsal horn lack sGC. Double labeling showed that neuronal nitric oxide synthase (nNOS) seldom colocalized with sGC, but nNOS-positive structures were frequently closely apposed to sGC-positive structures, suggesting that in the superficial dorsal horn NO acts mainly in a paracrine manner. Our data suggest that the NK1 receptor-positive projection neurons in lamina I are a major target of NO r...Continue Reading

References

Dec 7, 1992·Neuroscience Letters·N J DunL F Tseng
Dec 14, 1992·Neuroscience Letters·K F KittoG L Wilcox
Jul 8, 1992·The Journal of Comparative Neurology·J G ValtschanoffA Rustioni
Feb 21, 1994·Brain Research·P LiJ P Figueroa
Jan 1, 1994·Annual Review of Neuroscience·E M Schuman, D V Madison
Mar 1, 1994·Neuroscience·S SaitoD F Hanley
May 1, 1994·The European Journal of Neuroscience·R MorrisJ Garthwaite
Jan 1, 1995·Annual Review of Physiology·J Garthwaite, C L Boulton
Aug 18, 1993·Biochimica Et Biophysica Acta·H H SchmidtU Walter
Dec 1, 1995·Cell and Tissue Research·L G Eshkind, R E Leube
Nov 1, 1996·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K S Shindler, K A Roth
Dec 1, 1996·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B HunyadyE Mezey
Feb 5, 1999·Progress in Brain Research·R D HawkinsO Arancio
Feb 13, 1999·Progress in Neurobiology·M J Millan
Mar 3, 1999·The European Journal of Neuroscience·I A Belyantseva, G R Lewin
Oct 8, 1999·Biochemical and Biophysical Research Communications·J BudworthK Powell
Mar 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·T C BellamyJ Garthwaite
Nov 4, 2000·Current Review of Pain·Z D Luo, D Cizkova
May 24, 2001·Current Opinion in Neurology·L L Thomsen, J Olesen
Feb 19, 2003·Brain Research. Brain Research Reviews·William D Willis
Jul 4, 2003·British Journal of Pharmacology·Barry J GibbJohn Garthwaite
Dec 23, 2003·The International Journal of Biochemistry & Cell Biology·Hideaki Okamoto
Apr 6, 2004·The Journal of Comparative Neurology·Jin-Dong DingRichard J Weinberg
Jul 6, 2004·Neuroscience Letters·De-Yong Liang, J David Clark
Aug 18, 2004·Neurochemistry International·Doris KoeslingAndreas Friebe
Mar 26, 2005·The Journal of Comparative Neurology·Jin-Dong DingRichard J Weinberg

❮ Previous
Next ❯

Citations

May 6, 2009·Trends in Neurosciences·Achim SchmidtkoGerd Geisslinger
Aug 21, 2013·Saudi Journal of Biological Sciences·Faiza B Abdu
Nov 17, 2009·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Hwi-Seok JungDong-Ho Youn
Jan 29, 2021·The Journal of Biological Chemistry·Dennis J StuehrArnab Ghosh
May 4, 2021·British Journal of Pharmacology·Hannes SchmidtAchim Schmidtko

❮ Previous
Next ❯

Related Concepts

Related Feeds

Ataxias (MDS)

Ataxia is a neurological condition characterized by lack of voluntary coordination of muscle movements including loss of coordination, balance, and speech. Discover the latest research on ataxia here.

Ataxia

Ataxia is a neurological condition characterized by lack of voluntary coordination of muscle movements including loss of coordination, balance, and speech. Discover the latest research on ataxia here.