Localization of StarD5 cholesterol binding protein.

Journal of Lipid Research
Daniel Rodriguez-AgudoWilliam M Pandak

Abstract

Human StarD5 belongs to the StarD4 subfamily of START (for steroidogenic acute regulatory lipid transfer) domain proteins. We previously reported that StarD5 is located in the cytosolic fraction of human liver and binds cholesterol and 25-hydroxycholesterol. After overexpression of the gene encoding StarD5 in primary rat hepatocytes, free cholesterol accumulated in intracellular membranes. These findings suggested StarD5 to be a directional cytosolic sterol transporter. The objective of this study was to determine the localization of StarD5 in human liver. Western blot analysis confirmed StarD5's presence in the liver but not in human hepatocytes. Immunohistochemistry studies showed StarD5 localized within sinusoidal lining cells in the human liver and colocalized with CD68, a marker for Kupffer cells. Western blot analyses identified the presence of StarD5 in monocytes and macrophages as well as mast cells, basophils, and promyelocytic cells, but not in human hepatocytes, endothelial cells, fibroblasts, osteocytes, astrocytes, or brain tissue. Cell fractionation and immunocytochemistry studies on THP-1 macrophages localized StarD5 to the cytosol and supported an association with the Golgi. The presence of this cholesterol/25-h...Continue Reading

References

Apr 10, 1997·International Journal of Cancer. Journal International Du Cancer·C Moog-LutzM C Rio
May 14, 1999·Trends in Biochemical Sciences·C P Ponting, L Aravind
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Raymond E SoccioJan L Breslow
Jun 11, 2002·Nature Structural Biology·Steven L RoderickDavid E Cohen
May 2, 2003·The Journal of Biological Chemistry·Raymond E Soccio, Jan L Breslow
May 29, 2003·Molecular and Cellular Endocrinology·Jerome F StraussHisahiko Hiroi
Apr 6, 2004·Molecular and Cellular Endocrinology·Orsolya Dohán, Nancy Carrasco
Oct 7, 2004·Biochimica Et Biophysica Acta·Sushmita Mukherjee, Frederick R Maxfield
Nov 27, 2004·Biology of Reproduction·Cynthia GomesJaemog Soh
May 18, 2005·Journal of Lipid Research·Daniel Rodriguez-AgudoWilliam M Pandak
Jun 25, 2005·Journal of Cell Science·Fabien Alpy, Catherine Tomasetto

❮ Previous
Next ❯

Citations

May 29, 2009·American Journal of Physiology. Renal Physiology·Yu-Chyu ChenBarbara J Clark
Sep 29, 2012·Journal of Lipid Research·Danny LétourneauJean-Guy LeHoux
Apr 12, 2008·Journal of Lipid Research·Daniel Rodriguez-AgudoWilliam M Pandak
Oct 4, 2011·The Journal of Endocrinology·Barbara J Clark
Jan 21, 2014·The International Journal of Biochemistry & Cell Biology·Maria Calderon-DominguezDaniel Rodríguez-Agudo
Jan 4, 2013·Cellular and Molecular Life Sciences : CMLS·Bruno MesminGuillaume Drin
Mar 11, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Andres Gottfried-BlackmoreKaren Bulloch
Aug 22, 2007·Progress in Lipid Research·William A Prinz
May 8, 2015·Journal of Lipids·Ravi Kant Upadhyay
Jan 23, 2013·Molecular and Cellular Endocrinology·Danny LétourneauJean-Guy LeHoux
Dec 30, 2014·Molecular and Cellular Endocrinology·Danny LétourneauJean-Guy LeHoux
Jul 20, 2011·Biochimica Et Biophysica Acta·Daniel Rodriguez-AgudoWilliam M Pandak
Aug 31, 2006·Journal of Lipid Research·Wen-Jun WangDavid E Cohen
Sep 24, 2016·Clinical Science·Ugo Soffientini, Annette Graham
Feb 26, 2009·Médecine sciences : M/S·Fabien AlpyCatherine Tomasetto
Apr 25, 2019·Journal of Lipid Research·Daniel Rodriguez-AgudoGregorio Gil
Jan 14, 2020·Molecular and Cellular Endocrinology·Barbara J Clark

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Basophils

Basophils are myeloid cells with a high affinity IgE receptor and is involved in inflammatory responses during allergy. Discover the latest research on Basophils here.

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.