Abstract
Human StarD5 belongs to the StarD4 subfamily of START (for steroidogenic acute regulatory lipid transfer) domain proteins. We previously reported that StarD5 is located in the cytosolic fraction of human liver and binds cholesterol and 25-hydroxycholesterol. After overexpression of the gene encoding StarD5 in primary rat hepatocytes, free cholesterol accumulated in intracellular membranes. These findings suggested StarD5 to be a directional cytosolic sterol transporter. The objective of this study was to determine the localization of StarD5 in human liver. Western blot analysis confirmed StarD5's presence in the liver but not in human hepatocytes. Immunohistochemistry studies showed StarD5 localized within sinusoidal lining cells in the human liver and colocalized with CD68, a marker for Kupffer cells. Western blot analyses identified the presence of StarD5 in monocytes and macrophages as well as mast cells, basophils, and promyelocytic cells, but not in human hepatocytes, endothelial cells, fibroblasts, osteocytes, astrocytes, or brain tissue. Cell fractionation and immunocytochemistry studies on THP-1 macrophages localized StarD5 to the cytosol and supported an association with the Golgi. The presence of this cholesterol/25-h...Continue Reading
References
Oct 1, 1988·The Journal of Cell Biology·Y KubotaT J Lawley
Mar 24, 1995·Science·D LinW L Miller
Apr 10, 1997·International Journal of Cancer. Journal International Du Cancer·C Moog-LutzM C Rio
Sep 24, 1998·Biophysical Journal·S MukherjeeF R Maxfield
May 14, 1999·Trends in Biochemical Sciences·C P Ponting, L Aravind
Nov 5, 1999·Biochimica Et Biophysica Acta·D E CohenD R Beier
Sep 20, 2000·Biochemistry·H S BoseW L Miller
Dec 12, 2000·Biochemistry·L FengD E Cohen
Mar 29, 2001·Proteins·L M IyerL Aravind
Oct 30, 2001·The Journal of Biological Chemistry·Mingming HaoFrederick R Maxfield
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Raymond E SoccioJan L Breslow
Jun 11, 2002·Nature Structural Biology·Steven L RoderickDavid E Cohen
Oct 9, 2002·The Journal of Biological Chemistry·William M PandakPhillip Hylemon
May 2, 2003·The Journal of Biological Chemistry·Raymond E Soccio, Jan L Breslow
May 29, 2003·Molecular and Cellular Endocrinology·Jerome F StraussHisahiko Hiroi
Apr 6, 2004·Molecular and Cellular Endocrinology·Orsolya Dohán, Nancy Carrasco
Jun 25, 2004·The Journal of Biological Chemistry·Yankun LiIra Tabas
Sep 3, 2004·Journal of Lipid Research·Shunlin RenWilliam M Pandak
Oct 7, 2004·Biochimica Et Biophysica Acta·Sushmita Mukherjee, Frederick R Maxfield
Nov 27, 2004·Biology of Reproduction·Cynthia GomesJaemog Soh
Mar 12, 2005·The Journal of Biological Chemistry·Raymond E SoccioJan L Breslow
May 18, 2005·Journal of Lipid Research·Daniel Rodriguez-AgudoWilliam M Pandak
Jun 25, 2005·Journal of Cell Science·Fabien Alpy, Catherine Tomasetto
Citations
May 29, 2009·American Journal of Physiology. Renal Physiology·Yu-Chyu ChenBarbara J Clark
Dec 8, 2009·Journal of Lipid Research·Joshua J RiegelhauptJan L Breslow
Sep 29, 2012·Journal of Lipid Research·Danny LétourneauJean-Guy LeHoux
Oct 12, 2012·Journal of Lipid Research·Daniel Rodriguez-AgudoWilliam M Pandak
Apr 12, 2008·Journal of Lipid Research·Daniel Rodriguez-AgudoWilliam M Pandak
Oct 4, 2011·The Journal of Endocrinology·Barbara J Clark
Jan 21, 2014·The International Journal of Biochemistry & Cell Biology·Maria Calderon-DominguezDaniel Rodríguez-Agudo
Jan 4, 2013·Cellular and Molecular Life Sciences : CMLS·Bruno MesminGuillaume Drin
Mar 11, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Andres Gottfried-BlackmoreKaren Bulloch
Aug 22, 2007·Progress in Lipid Research·William A Prinz
Apr 23, 2013·Disasters·Mark Kammerbauer
Jun 4, 2014·Traffic·Andrew Midzak, Vassilios Papadopoulos
Oct 16, 2014·Journal of Hepatology·Otto StraussAnthony Phillips
Jan 15, 2015·Emergency Medicine Australasia : EMA·Sultan Al-ShaqsiAbdullah Al-Harthy
May 25, 2010·Clinical Science·Faye BorthwickAnnette Graham
May 8, 2015·Journal of Lipids·Ravi Kant Upadhyay
Jan 23, 2013·Molecular and Cellular Endocrinology·Danny LétourneauJean-Guy LeHoux
Dec 30, 2014·Molecular and Cellular Endocrinology·Danny LétourneauJean-Guy LeHoux
Jul 23, 2013·Biochimica Et Biophysica Acta·Danny LétourneauJean-Guy LeHoux
Jul 20, 2011·Biochimica Et Biophysica Acta·Daniel Rodriguez-AgudoWilliam M Pandak
Aug 31, 2006·Journal of Lipid Research·Wen-Jun WangDavid E Cohen
Sep 16, 2006·Molecular Endocrinology·Walter L Miller
Sep 24, 2016·Clinical Science·Ugo Soffientini, Annette Graham
Feb 26, 2009·Médecine sciences : M/S·Fabien AlpyCatherine Tomasetto
Apr 25, 2019·Journal of Lipid Research·Daniel Rodriguez-AgudoGregorio Gil
Dec 5, 2020·Oxidative Medicine and Cellular Longevity·Qin CaoQifa Ye
Jan 14, 2020·Molecular and Cellular Endocrinology·Barbara J Clark