Localization of steroidogenic enzymes in macaque luteal tissue during the menstrual cycle and simulated early pregnancy: immunohistochemical evidence supporting the two-cell model for estrogen production in the primate corpus luteum

Biology of Reproduction
S L Sanders, R L Stouffer

Abstract

It is hypothesized that the two-cell model for estrogen production by the ovarian follicle is preserved in the primate corpus luteum, but there is little direct evidence to support this theory. To determine the sites of androgen and estrogen synthesis within the primate corpus luteum and to ascertain whether changes in steroid hormone levels are related to steroidogenic enzyme expression, the enzymes converting progesterone to androgen (cytochrome P450 17alpha-hydroxylase/17,20 lyase; P450(c17)) and then to estrogen (aromatase; P450(arom)), as well as P450 side-chain cleavage (P450(scc)) and 3beta-hydroxysteroid dehydrogenase (3beta HSD), were detected by immunohistochemistry in macaque luteal tissue throughout the menstrual cycle and simulated early pregnancy. Corpora lutea were collected from rhesus monkeys in the early (Days 2-4 post-LH surge), mid (Days 6-8), mid-late (Days 10-12), and late (Days 14-15) luteal phase and after 1, 3, 6, or 9 days of hCG treatment that began on Day 9 of the luteal phase. Specific cytoplasmic staining for P450(c17), P450(arom), P450(scc), and 3beta HSD was present in luteal cells, but not in the microvasculature, within all luteal tissues examined. P450(c17)-stained luteal cells were located al...Continue Reading

Citations

Mar 15, 2008·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Ana Alonso MartínezPilar Molist García
Jun 14, 2002·Journal of Reproductive Immunology·Luigi DevotoAlberto Palomino
Mar 20, 2002·Molecular and Cellular Endocrinology·Luigi DevotoJerome F Strauss
Nov 10, 2011·Human Reproduction Update·Angela R BaerwaldRoger A Pierson
Nov 3, 2010·Molecular Human Reproduction·Fuhua XuBernhard Lindenthal
Nov 14, 2003·Reproductive Biology and Endocrinology : RB&E·Lane K Christenson, Luigi Devoto
Apr 11, 2012·Acta Histochemica Et Cytochemica·Mayu HanaueKen Takamatsu
Aug 26, 2011·Climacteric : the Journal of the International Menopause Society·K F EthunS E Appt
May 6, 2009·Reproductive Biomedicine Online·Luigi DevotoJerome F Strauss
Feb 6, 2010·Reproductive Biomedicine Online·I E MessinisK Dafopoulos
Jun 9, 2009·Toxicology and Applied Pharmacology·Jochen ReinsbergDietrich Klingmueller
Feb 24, 2015·Frontiers in Endocrinology·Patrick R Hannon, Jodi A Flaws
May 8, 2016·The Journal of Steroid Biochemistry and Molecular Biology·Ian M Bird, David H Abbott
Jun 22, 2000·Biology of Reproduction·B D Murphy
Sep 15, 2006·Journal of the Royal Society, Interface·Robert G ClydeJohn W Crawford

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