Localization of synapsin-I and PSD-95 in developing postnatal rat cerebellar cortex

Brain Research. Developmental Brain Research
Orlando J CastejónMichael E Dailey

Abstract

The comparative localization of two prominent synaptic proteins, synapsin-I (Syn-I) and PSD-95, was investigated in slices of developing (P3-P21) rat cerebellar cortex using double- or triple-label fluorescence immunohistochemistry and confocal microscopy. During the first postnatal week, Syn-I and PSD-95 immunoreactive (IR) puncta were strongly concentrated in the Purkinje cell layer (PCL) where they circumscribed irregularly shaped PC somata, forming pericellular nests that likely correspond to early climbing fiber synapses. PSD-95 and Syn-I puncta also were found along the shafts and at the tips of growing PC dendrite branches labeled with calbindin. During the second postnatal week, synaptic puncta were lost from the PC layer, while many new puncta were added to the molecular layer (ML). At P10, about half of the PCs were circumscribed by PSD-95 or Syn-I puncta, whereas at P14 no PCs were circumscribed. By P14, PSD-95 and Syn-I became most strongly localized to many small puncta in the ML and to large clusters at mossy fiber rosettes in the glomerular layer (GL) where PSD-95 often encircled Syn-I clusters. Some large clusters in the GL contained only PSD-95 or Syn-I, but not both, suggesting differential growth or remodelin...Continue Reading

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Citations

Nov 26, 2010·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Zhi-Wei ZhuDing-Wen Wu
Apr 20, 2010·Toxicology·Takashi IwakuraRitsuko Ohtani-Kaneko
Nov 10, 2009·Neuroscience Research·Ritsuko Ohtani-KanekoChiho Watanabe
Sep 16, 2009·Brain Research Bulletin·Mingshan LiJinbo Deng
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May 20, 2008·Biophysical Journal·Sherry-Ann BrownLeslie M Loew
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Nov 20, 2019·Journal of Molecular Neuroscience : MN·Martina Baliova, Frantisek Jursky
Nov 26, 2009·Behavioural Brain Research·Regina Dahlhaus, Alaa El-Husseini

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