PMID: 9523698Apr 2, 1998Paper

Localization of the 23-kDa subunit of the oxygen-evolving complex of photosystem II by electron microscopy

European Journal of Biochemistry
Egbert J BoekemaJ Barber

Abstract

A dimeric photosystem II light-harvesting II super complex (PSII-LHCII SC), isolated by sucrose density gradient centrifugation, was previously structurally characterized [Boekema, E. J., Hankamer, B., Bald, D., Kruip, J., Nield, J., Boonstra, A. F., Barber, J. & Rögner, M. (1995) Proc. Natl Acad. Sci. USA 92, 175-179]. This PSII-LHCII SC bound the 33-kDa subunit of the oxygen-evolving complex (OEC), but lacked the 23-kDa and 17-kDa subunits of the OEC. Here the isolation procedure was modified by adding 1 M glycine betaine (1-carboxy-N,N,N-trimethylmethanaminium hydroxide inner salt) to the sucrose gradient mixture. This procedure yielded PSII-LHCII SC that contained both the 33-kDa and the 23-kDa subunits and had twice the oxygen-evolving capacity of the super complexes lacking the 23-kDa polypeptide. Addition of CaCl2 to PSII-LHCII SC with the 23-kDa subunit attached did not increase the oxygen-evolution rate. This suggests that the 23-kDa subunit is bound in a functional manner and is present in significant amounts. Over 5000 particle projections extracted from electron microscope images of negatively stained PSII-LHCII SC, isolated in the presence and absence of glycine betaine, were analyzed using single-particle image-av...Continue Reading

Citations

Apr 14, 2011·Photosynthesis Research·Cristina PaglianoJames Barber
Jun 14, 2005·Micron : the International Research and Review Journal for Microscopy·Frantisek VachaMartin Vacha
Aug 17, 1999·Current Opinion in Structural Biology·J Barber, W Kühlbrandt
Sep 27, 2003·European Journal of Biochemistry·Mónica BalseraJavier De Las Rivas
Jul 18, 2012·The Journal of Biological Chemistry·Ryutaro TokutsuJun Minagawa
Dec 24, 2015·Frontiers in Plant Science·Patrycja HaniewiczDario Piano
Feb 15, 2011·Journal of Photochemistry and Photobiology. B, Biology·Hana Popelkova, Charles F Yocum
Apr 11, 2003·Journal of Plant Physiology·Suleyman I AllakhverdievRobert Carpentier
Feb 24, 2015·The Plant Journal : for Cell and Molecular Biology·Jun Minagawa, Ryutaro Tokutsu
Jun 19, 1998·Biochimica Et Biophysica Acta·M K Lyon
Dec 29, 2004·Biochimica Et Biophysica Acta·Jan P Dekker, Egbert J Boekema
Nov 30, 2010·Biochimica Et Biophysica Acta·Roman KouřilEgbert J Boekema
Nov 24, 2001·The Journal of Biological Chemistry·Maria SchubertThomas Kieselbach
Aug 7, 1998·Biochimica Et Biophysica Acta·J Barber
Apr 27, 1999·Biochimica Et Biophysica Acta·F A WollmanR Nechushtai

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