PMID: 7029211Jan 1, 1981Paper

Localization of the 3' end of 16S rRNA in Escherichia coli 30S ribosomal subunits by immuno electron microscopy

Molecular & General Genetics : MGG
R LührmannG Stöffler

Abstract

The location of the 3' end of 16S rRNA in E. coli 30S ribosomal subunits has been determined by immuno electron microscopy. The 3' terminal adenosine of isolated 16S rRNA was oxidized with sodium periodate and reacted with N-gamma-(2,4-dinitrophenyl) aminobutyric acid hydrazide. Functionally active 30S subunits were reconstituted from DNP-16S rRNA and total 30S ribosomal proteins. DNP-30S subunits were complexed with DNP-specific IgG-antibody and examined in the electron microscope. The 3' end of the 16S rRNA was mapped at a single region located at the inner side of the large lobe of the 30S subunit. The location of the 3' end also provides information as to the topography of the binding domain of natural mRNA on 30S subunits, since a pyrimidine-rich sequence at the 3' terminal region of 16S rRNA participates in the correct alignment of natural mRNAs during initiation complex formation.

References

Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J J DunnF W Studier
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S M Politz, D G Glitz
Aug 1, 1977·Archives of Biochemistry and Biophysics·J W HersheyJ L Fakunding
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M Keren-ZurJ Ofengand
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H M Olson, D G Glitz
Oct 15, 1975·FEBS Letters·A P CzernilofskyG Stöffler
Aug 13, 1974·Biochemistry·R M D'Alisa, B F Erlanger
Jun 1, 1974·Nucleic Acids Research·S A Reines, C R Cantor
Jun 1, 1960·Proceedings of the National Academy of Sciences of the United States of America·P C ZamecnikJ F Scott
Mar 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·J W Hershey, R E Thach

❮ Previous
Next ❯

Citations

Jul 11, 1985·Nucleic Acids Research·P Spitnik-ElsonR Abramowitz
Jul 5, 1985·Journal of Molecular Biology·A Expert-Bezançon, P L Wollenzien
Apr 2, 2017·G3 : Genes - Genomes - Genetics·Akram AbolbaghaeiXuhua Xia
Jan 1, 1986·Progress in Biophysics and Molecular Biology·K Nagano, M Harel
Sep 17, 1988·Journal of Theoretical Biology·K NaganoM Takezawa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.