PMID: 7539283Apr 1, 1995Paper

Localization of the 8;13 translocation breakpoint associated with myeloproliferative disease to a 1.5 Mbp region of chromosome 13

Genes, Chromosomes & Cancer
H KempskiJ K Cowell

Abstract

There are five reported cases of an atypical myeloproliferative disorder in which the leukemia cells have a consistent t(8;13)(p11;q12) translocation. We analyzed the breakpoint in metaphases from two of these patients by fluorescence in situ hybridization using a series of yeast artificial chromosomes (YACs) derived from the 13q12 region. We found that a YAC containing the FLT1 and FLT3 oncogenes was localized distal to the 13q12 breakpoint and was not rearranged. YAC66, a YAC that lies immediately adjacent to the chromosome 13 centromere, was localized proximal to the 13q12 breakpoint and was not rearranged. A third YAC, which is located between FLT1 and YAC66, was unrearranged in normal metaphase chromosomes, but showed hybridization signals on both derivative chromosomes in both cases. Thus, the breakpoints in these two cases are localized to the same 1.5 Mbp region of 13q12. This may be the site of an unidentified gene involved in the pathogenesis of some types of leukemia.

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Citations

Dec 31, 1997·Genes, Chromosomes & Cancer·R C AguiarN C Cross
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·I H StillJ K Cowell
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·C PopoviciM J Pébusque
Jun 22, 1999·Medizinische Klinik·A ReitherN C Cross
Apr 6, 2001·Blood·K OkudaJ D Griffin
Nov 15, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·N A HeeremaF M Uckun

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