Localization of the functional domains of human tissue inhibitor of metalloproteinases-3 and the effects of a Sorsby's fundus dystrophy mutation.

The Journal of Biological Chemistry
K P LangtonNorman McKie

Abstract

A transient COS-7 cell expression system was used to investigate the functional domain arrangement of tissue inhibitor of metalloproteinases-3 (TIMP-3), specifically to assess the contribution of the amino- and carboxyl-terminal domains of the molecule to its matrix metalloproteinase (MMP) inhibitory and extracellular matrix (ECM) binding properties. Wild type TIMP-3 was entirely localized to the ECM in both its glycosylated (27 kDa) and unglycosylated (24 kDa) forms. A COOH-terminally truncated TIMP-3 molecule was found to be a non-ECM bound MMP inhibitor, whereas a chimeric TIMP molecule, consisting of the NH2-terminal domain of TIMP-2 fused to the COOH-terminal domain of TIMP-3, displayed ECM binding, albeit with a lower affinity than the wild type TIMP-3 molecule. Thus the functional domain arrangement of TIMP-3 is analogous to that seen in TIMP-1 and -2, namely that the NH2-terminal domain is responsible for MMP inhibition whereas the COOH-terminal domain is most important in mediating the specific functions of the molecule. A mutant TIMP-3 in which serine 181 was changed to a cysteine, found in Sorsby's fundus dystrophy, a hereditary macular degenerative disease, was also expressed in COS-7 cells. This gave rise to an add...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T T Yang, S P Hawkes
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T C BooneK E Langley
Jun 1, 1990·The Biochemical Journal·R A WilliamsonR B Freedman
Dec 1, 1989·Ophthalmology·M R CaponA C Bird
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G I GoldbergC S He
Dec 1, 1989·Ophthalmology·P J PolkinghorneA C Bird
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A Aruffo, B Seed
Sep 1, 1995·Nature Genetics·S G JacobsonE M Stone
Jan 1, 1995·Methods in Enzymology·G Murphy, F Willenbrock
Nov 1, 1993·Journal of Cellular Physiology·A N MurphyW G Stetler-Stevenson
Jun 1, 1996·Seminars in Cancer Biology·W G Stetler-StevensonM Corcoran
Nov 29, 1996·The Journal of Biological Chemistry·J GreeneY E Shi
Jan 1, 1996·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·B Anand-ApteS S Apte
May 1, 1997·Journal of Protein Chemistry·D A DouglasQ A Sang
Mar 20, 1998·Journal of Clinical Pharmacology·D C Celentano, W H Frishman
Feb 1, 1949·The British Journal of Ophthalmology·A SORSBY, M E J MASON

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Citations

May 9, 2006·Cancer Metastasis Reviews·Rosemarie ChircoHyeong-Reh Choi Kim
Mar 10, 2000·Biochimica Et Biophysica Acta·K BrewH Nagase
May 15, 2002·Brain Research. Molecular Brain Research·E M MuirJ H Rogers
Mar 22, 2002·Progress in Retinal and Eye Research·Jeremy M Sivak, M Elizabeth Fini
Oct 28, 2004·The Journal of Molecular Diagnostics : JMD·Wei-Cheng XueAnnie N Y Cheung
Apr 12, 2011·Nature Neuroscience·Pilar EstevePaola Bovolenta
May 30, 2009·The Journal of Biological Chemistry·Jian Hua QiBela Anand-Apte
Nov 23, 2012·Molecular Human Reproduction·Yuqian XiangXinzhi Zhao
Dec 17, 2002·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Martin J Tymms
Oct 9, 2003·Annals of the Rheumatic Diseases·F F MohammedR Khokha
Jul 14, 2006·The British Journal of Ophthalmology·M A MajidA D Dick
Mar 27, 2007·The British Journal of Ophthalmology·Mohammed A MajidAndrew D Dick
Jun 6, 2000·The British Journal of Ophthalmology·C S SethiN H Chong
May 12, 2012·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M A NieuwesteegS Damjanovski
Nov 1, 2001·Annual Review of Cell and Developmental Biology·M D Sternlicht, Z Werb
Aug 8, 2009·American Journal of Respiratory and Critical Care Medicine·Hitesh S DeshmukhGeorge D Leikauf
Jun 1, 2013·Parasites & Vectors·Cinzia CantacessiAlex Loukas
Jul 1, 2011·Heart Failure Reviews·Linn MooreZamaneh Kassiri
Oct 13, 2006·The Journal of Cell Biology·W Brian SaundersGeorge E Davis
Apr 19, 2003·Experimental Dermatology·E Kerkelä, U Saarialho-Kere
Feb 26, 2000·Annual Review of Genetics·A RattnerJ Nathans
Jan 6, 2015·Apoptosis : an International Journal on Programmed Cell Death·Jian Hua Qi, Bela Anand-Apte
Sep 23, 2006·American Journal of Ophthalmology·Ruth J LinDouglas Vollrath
Jun 20, 2014·Biochemical and Biophysical Research Communications·Fang YanWeiyao Chen
Feb 3, 2018·International Journal of Molecular Sciences·Ruth M EscalonaNuzhat Ahmed
Jan 23, 1999·The Journal of Biological Chemistry·G BorlandA Ager
Aug 22, 2018·Journal of Neuroscience Research·Bela Anand-ApteHeidi Stöhr

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