Localization of the GLUT1 glucose transporter to brefeldin A-sensitive vesicles of differentiated CIT3 mouse mammary epithelial cells

Cell Biology International
P M Haney

Abstract

Glucose is a precursor of lactose, the major carbohydrate and osmotic constituent of human milk, which is synthesized in the Golgi. The GLUT1 glucose transporter is the only glucose transporter isoform expressed in the mammary gland. The hypothesis that lactogenic hormones induce GLUT1 and cause its localization to the Golgi of mammary epithelial cells was tested in CIT(3)mouse mammary epithelial cells. Treatment with prolactin and hydrocortisone caused a 15-fold induction of GLUT1 by Western blotting, but 2-deoxyglucose uptake decreased. Subcellular fractionation and density gradient centrifugation demonstrated enrichment of Golgi fractions with GLUT1. Lactogenic hormones enhanced GLUT1 glycosylation, but did not determine whether GLUT1 was targeted to plasma membrane or to Golgi. Confocal microscopy revealed that lactogenic hormones alter GLUT1 targeting from a plasma membrane pattern to a predominant perinuclear distribution with punctate scattering through the cytoplasm. GLUT1 is targeted to a compartment which is more sensitive to Brefeldin A than the compartments in which GM130 and beta-COP reside. Targeting of GLUT1 to endosomes was specifically excluded. We conclude that prolactin and hydrocortisone induce GLUT1, enhanc...Continue Reading

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Citations

Apr 23, 2003·Advanced Drug Delivery Reviews·James L McManaman, Margaret C Neville
Apr 23, 2003·Cancer Letters·Suzanne RogersJames D Best
Dec 1, 2009·Animal : an International Journal of Animal Bioscience·S Truchet, M Ollivier-Bousquet
Nov 14, 2013·Journal of Mammary Gland Biology and Neoplasia·Feng-Qi Zhao
Jan 7, 2005·American Journal of Physiology. Cell Physiology·Shannon L Kelleher, Bo Lönnerdal
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Jan 1, 2015·American Journal of Physiology. Endocrinology and Metabolism·J S WattezF Pinet
May 2, 2012·The Biochemical Journal·Nancy ZaarourRoland Govers

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