Localization of the human SH-protease inhibitor in the epidermis. Immunofluorescent studies

Acta histochemica
O RäsänenA Rinne

Abstract

Human epidermis contains a low molecular weight SH-protease inhibitor (Human Epidermal Inhibitor = HEI), whose epidermal localization was performed with the indirect immunofluorescence method. The fluorescence was most intensive in the cytoplasms of epidermal cells, often occurring perinuclearly. The fluorescent material in the frozen sections was often finely granular and occasionally extended outside the cytoplasm, while the fluorescence in fixed sections was more uniform, but weaker. Stratum basale generally stained poorly or not at all, as did also stratum lucidum. Stratum corneum stained fairly intensively throughout. In addition to fixation, the outcome of staining was also affected by the thickness of the epidermis, particularly stratum corneum. The significance of this inhibitor for the differentiation of epidermal cells and the keratinization of epidermis has therefore been discussed, and the authors assume it to be of considerable significance in these processes.

References

Jan 1, 1976·Acta Chemica Scandinavica. Series B: Organic Chemistry and Biochemistry·M Järvinen

Citations

Jan 1, 1984·Histochemistry·M E Davies, A J Barrett
Jan 1, 1990·Acta histochemica·H PernuM Järvinen
Aug 11, 1987·Nucleic Acids Research·T KartasovaP van de Putte
Feb 7, 2013·Journal of Cancer·Spela Magister, Janko Kos
Jun 1, 1981·The British Journal of Dermatology·V K Hopsu-HavuM Järvinen
Jan 1, 1994·European Journal of Vascular Surgery·T JuvonenO Niemelä
Sep 1, 1994·The Anatomical Record·K O SöderströmA Rinne
May 12, 2018·Cancer Immunology, Immunotherapy : CII·Janko KosAnahid Jewett
Feb 12, 2020·Journal of Biochemical and Molecular Toxicology·Yue ChenYong Xu
Jul 4, 1995·International Journal of Cancer. Journal International Du Cancer·K O SöderströmA Rinne

Related Concepts

Protoplasm
Epidermis
Immunofluorescence Assay
Protease Inhibitors

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