Localization of the in vivo expression of P and F1 fimbriae in chickens experimentally inoculated with pathogenic Escherichia coli

Microbial Pathogenesis
S A PourbakhshJ M Fairbrother

Abstract

Escherichia coli causing septicemia in poultry often possess F1 (type 1) and/or P fimbriae which may be involved in bacterial colonization and infection. To investigate the expression of these fimbriae in vivo, two pathogenic E. coli strains with different fimbrial profiles, TK3 (fim+/pap+) and MT78 (fim+/pap-), were administered to 2-week-old chickens by either the intratracheal or caudal thoracic air sac inoculation route. Antibodies specific for native F1 fimbriae were detected by ELISA and immunodot in the serum of chickens inoculated with either strain MT78 or strain TK3, irrespective of the route of inoculation. Antibodies specific for P fimbriae of serotype F11 were detected by ELISA and immunoblotting in the serum of chickens inoculated by either route with strain TK3. F1, but not P fimbriae, were expressed by bacteria colonizing the trachea of chickens inoculated by the air sac route with strain MT78 or TK3, as demonstrated by examination of frozen tissue sections using immunofluorescence. F1 fimbriae were also expressed by bacteria colonizing the air sacs and lungs, but not by bacteria in the blood or other internal organs, of chickens inoculated with either strain. P fimbriae were expressed by bacteria colonizing the...Continue Reading

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Citations

Sep 30, 2005·Avian Pathology : Journal of the W.V.P.A·Fréderic VandemaeleBruno Maria Goddeeris
Jul 25, 2006·Infection and Immunity·Christopher D HerrenSuman Mukhopadhyay
Dec 22, 2005·Infection and Immunity·Subhashinie KariyawasamLisa K Nolan
Sep 24, 2004·Journal of Molecular Microbiology and Biotechnology·F VerdonckB M Goddeeris
Feb 9, 2012·Avian Diseases·Subhashinie Kariyawasam, Lisa K Nolan
Mar 3, 2010·International Journal of Environmental Research and Public Health·S M Lutful Kabir
Apr 5, 2003·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·W D da SilveiraM Brocchi
Dec 17, 2014·Veterinary Medicine International·Moemen A MohamedElshimaa Rafeek
Nov 27, 2004·Comparative Immunology, Microbiology and Infectious Diseases·Maria A R MonroyAntonio J Piantino Ferreira
Feb 16, 2017·Research in Veterinary Science·Hassan M A HejairHuochun Yao
Aug 1, 2018·Environmental Microbiology·Letícia B MatterJorge A Girón
Jun 19, 2012·Journal of Veterinary Science·Yong-Wun JeongHyuk-Joon Kwon
Aug 10, 2020·Applied and Environmental Microbiology·Aamir AliPeter Schierack
Apr 9, 2005·Microbiology·Pierre GermonMaryvonne Moulin-Schouleur
Jan 8, 2021·Avian Pathology : Journal of the W.V.P.A·Andreas AlberLonneke Vervelde

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