Localization of TIMP-1, TIMP-2, TIMP-3, gelatinase A and gelatinase B in pathological human corneas

Current Eye Research
M C KenneyD J Brown

Abstract

Determine the tissue distribution patterns for tissue inhibitors of metalloproteinases (TIMP-1, TIMP-2, TIMP-3), gelatinase A and gelatinase B in normal and pathologic corneas. Corneas were examined by immunohistochemistry, using antibodies to TIMP-1, TIMP-2, TIMP-3, gelatinase A or gelatinase B. In normal corneas, TIMP-1 antibody stained the epithelium and endothelium. TIMP-2 and TIMP-3 stained the epithelium, keratocytes and endothelium. Gelatinase A staining was weak and restricted to the epithelial cells. Radial keratotomy scars showed increased staining for TIMP-1 and TIMP-2 around the epithelial cell plug and along the incision. Bullous keratopathy corneas showed TIMP staining patterns similar to normal corneas and increased gelatinase A staining in regions of subepithelial fibrosis. Stromal scars of keratoconus corneas also had increased staining with TIMP-1 and TIMP-2 antibodies. In many keratoconus corneas, the TIMP-3 staining pattern was similar to normal corneas. However, in some keratoconus corneas, when Bowman's layer was missing, the stroma beneath was completely devoid of TIMP-3 antibody staining. No gelatinase B was seen in either the normal or diseased corneas. These data suggest that TIMP-1 and TIMP-2 are impo...Continue Reading

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Citations

Feb 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Rajeev BuddiDonald J Brown
Jan 10, 2002·Clinical & Experimental Ophthalmology·S A Collier
Apr 25, 2006·Cornea·Hannu P S SeppäläYrjö T Konttinen
Apr 9, 2010·Current Eye Research·Sivaraman A BalasubramanianMark D P Willcox
Mar 2, 2019·Investigative Ophthalmology & Visual Science·Beau J FennerJodhbir S Mehta
Jul 17, 2004·Cornea·Batool JafriR Doyle Stulting
Jan 23, 2013·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Isabella M Y CheungTrevor Sherwin
Jun 19, 2016·Photochemistry and Photobiology·Taras ArdanJan Motlík
Apr 27, 2001·Cornea·M C KenneyD J Brown
Jan 15, 2004·Eye & Contact Lens·Charles R EdmondsBart Pemberton
Dec 25, 2004·Infection and Immunity·Marie Mei LeePaul D Soloway
Nov 30, 2006·Cornea·Raja NarayananM Cristina Kenney
Jun 15, 2007·Veterinary Ophthalmology·F J OllivierD E Brooks
Sep 5, 2013·Expert Opinion on Drug Metabolism & Toxicology·Clara C Chan, Victoria Squissato
Feb 18, 2014·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Isabella M Y CheungTrevor Sherwin
Mar 22, 2002·Progress in Retinal and Eye Research·Jeremy M Sivak, M Elizabeth Fini
Jun 8, 2002·Survey of Ophthalmology·Tina T L WongPeng T Khaw
Feb 16, 2002·Experimental Eye Research·T A BaileyN H Chong
Sep 5, 2002·Progress in Retinal and Eye Research·Haydee Bazan, Paulo Ottino
Apr 20, 2004·Progress in Retinal and Eye Research·Nick Di GirolamoDenis Wakefield
Mar 7, 2006·Biochimica Et Biophysica Acta·V A SmithS D Cook

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