Localization of tissue plasminogen activator mRNA in the developing rat cerebellum and effects of inhibiting tissue plasminogen activator on granule cell migration

Journal of Neurobiology
J H WareR N Pittman

Abstract

Tissue plasminogen activator (tPA) mRNA was localized in the developing cerebellum and the potential role of tPA in migration of cerebellar granule cells was investigated. Proteolytic assays and Northern blots showed little variation in levels of tPA proteolytic activity or tPA mRNA expression in the developing cerebellum. The distribution of cerebellar tPA mRNA at different ages was visualized by in situ hybridization histochemistry. At postnatal day 7 (P7), most labeled cells were in the internal granule layer or developing white matter, and very few if any premigratory granule cells contained tPA mRNA. Although the molecular layer contained labeled cells at all ages, cell counts indicated that a greater percentage of cells in the molecular layer contained tPA mRNA during adulthood than during the period of granule cell migration. The most striking change in tPA mRNA expression was in Purkinje neurons, most of which began to express tPA mRNA between P7 and P14. The potential role of tPA in granule cell migration was investigated by performing migration assays in cerebellar slice explants in the presence or absence of protease inhibitors. The presence of inhibitors did not affect the distance that granule cells migrated. Data ...Continue Reading

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Citations

Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·N W SeedsS P Haffke
Jun 9, 2009·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Nobuo Nagai, Osamu Matsuo
Jul 21, 2004·Trends in Cardiovascular Medicine·Manuel Yepes, Daniel A Lawrence
Jun 8, 2017·Brain Sciences·Ludovic GalasHitoshi Komuro
Nov 27, 2004·Experimental Biology and Medicine·Manuel Yepes, Daniel A Lawrence
Apr 16, 1998·Biochimica Et Biophysica Acta·K ProbaP Sonderegger
Oct 2, 2004·Stroke; a Journal of Cerebral Circulation·Xiaoying WangEng H Lo

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