Localization of TMC1 and LHFPL5 in auditory hair cells in neonatal and adult mice

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Xiaofen LiPingbo Huang

Abstract

The channel that governs mechanotransduction (MT) by hair cells in the inner ear has been investigated intensively for 4 decades, but its precise molecular composition remains enigmatic. Transmembrane channel-like protein 1 (TMC1) was recently identified as a component of the MT channel, and lipoma HMGIC fusion partner-like 5 (LHFPL5) is considered to be part of the MT complex and may functionally couple the tip link to the MT channel. As components of the MT complex, TMC1 and LHFPL5 are expected to localize at the lower end of the tip link in hair cells, a notion generally supported by previous studies on neonatal mice. However, the localization of these 2 proteins, particularly in the hair cells of adult mice, remains incompletely elucidated. Because determination of TMC1 and LHFPL5 localization at distinct developmental stages is essential for understanding their function and regulation, we used several approaches to examine the localization of these proteins in neonatal and adult hair cells in the mouse. We report several notable findings: 1) TMC1 and LHFPL5 predominantly localize at the tip of the shorter rows of stereocilia in neonatal hair cells, which largely verifies the previously published findings in neonatal hair c...Continue Reading

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Citations

Feb 11, 2020·Frontiers in Molecular Neuroscience·Feng TangWei Yuan
Nov 11, 2020·Proceedings of the National Academy of Sciences of the United States of America·Xiaojie YuPingbo Huang
Jan 26, 2021·Frontiers in Cell and Developmental Biology·Shaoyuan ZhuBrian M McDermott
Apr 20, 2021·Cellular and Molecular Life Sciences : CMLS·Shuang LiuWei Xiong

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