PMID: 2503927Jan 1, 1989Paper

Localization of transthyretin-mRNA and of immunoreactive transthyretin in the human fetus

Virchows Archiv. A, Pathological Anatomy and Histopathology
B Jacobsson

Abstract

Five human fetuses at mid-term (16-20 weeks) and one with a gestational age of 8 weeks were investigated. The cellular localization of transthyretin (TTR)-mRNA in different organs was demonstrated by in situ hybridization with a 35S-labelled, single-stranded RNA probe. Immunoreactive TTR (TTR-IR) was localized with a monoclonal antibody to TTR. In all fetuses, choroid plexus epithelial cells demonstrated intense labelling for TTR-mRNA as well as strong TTR-IR. Hepatocytes, on the other hand, showed weak in situ labelling and weak or, in some cases, non-demonstrable TTR-IR. In all mid-term fetuses, but not in the 8 week fetus, TTR-mRNA and TTR were also expressed in pancreatic endocrine A-cells. The degree of in situ labelling in these A-cells was moderate, whereas that of TTR-IR was strong. Despite negative findings for TTR-mRNA in the gut and the kidney, endocrine cells of the gut and epithelial cells of the renal proximal convoluted tubules showed TTR-IR in some of the fetuses. The investigation provides evidence for TTR synthesis in the human fetal choroid plexus, liver and endocrine pancreas. However, further studies are required to demonstrate TTR synthesis in the gut and the kidney.

References

Aug 1, 1978·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S HayashiG M Tener
Jan 1, 1989·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B JacobssonA Carlström
Jan 1, 1985·Virchows Archiv. A, Pathological Anatomy and Histopathology·H D GrayC H Horne
Jun 28, 1985·Biochemical and Biophysical Research Communications·M R WallaceM D Benson
Jul 1, 1986·Journal of Biochemistry·S WakasugiK Shimada
Mar 15, 1988·Biochemical and Biophysical Research Communications·R L MartoneE A Schon
Aug 1, 1988·Developmental Biology·T ThomasM Dziadek
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D R SopranoD S Goodman
Jan 1, 1987·Annual Review of Physiology·J H Dussault, J Ruel
Dec 1, 1986·Scandinavian Journal of Clinical and Laboratory Investigation·V P CollinsA Carlström
Feb 1, 1987·Journal of Clinical Pathology·G G CornwellP Westermark
Jun 16, 1982·Biochimica Et Biophysica Acta·P Felding, G Fex

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Citations

Jan 1, 1991·Neuromuscular Disorders : NMD·M J Saraiva
May 15, 2013·Placenta·K A LandersK Richard
Mar 19, 2011·Trends in Endocrinology and Metabolism : TEM·Jatin PatelKerry Richard
Oct 7, 2008·Experimental Neurology·Mónica Mendes Sousa, Maria João Saraiva
Oct 1, 2013·Biochimica Et Biophysica Acta·Roberta CascellaCristina Cecchi
Sep 29, 2011·Placenta·J PatelK Richard
Nov 17, 2010·Experimental Eye Research·Rachida A BouhenniDeepak P Edward
Jan 9, 2010·Annual Review of Pharmacology and Toxicology·Jean-Pierre Changeux
Apr 5, 2002·The Journal of Clinical Endocrinology and Metabolism·Rosa M CalvoGabriella Morreale de Escobar
Jul 9, 2004·The Journal of Clinical Endocrinology and Metabolism·Monique H A KesterGabriella Morreale de Escobar
Apr 30, 2009·The Journal of Clinical Endocrinology and Metabolism·Kelly A LandersKerry Richard
Aug 1, 2018·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Claire Sergent
May 10, 2017·Cellular and Molecular Life Sciences : CMLS·Seyyed Abolghasem GhadamiFabrizio Chiti

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