Localization of trinucleotide repeat sequences in myotonic dystrophy cells using a single fluorochrome-labeled PNA probe

BioTechniques
K L Taneja

Abstract

A labeled peptide nucleic acid (PNA) antisense probe was used to study the spatial distribution of triplet repeats (CTG) in human myotonic dystrophy (DM) cells by high-resolution fluorescence in situ hybridization (FISH). It was found that transcripts containing triplet repeats were present as a number of discrete foci in the DM nuclei. Greater numbers of foci were visible with the PNA probe than a comparable DNA probe. The PNA probe was also used to visualize the triplet-repeat expansion within the DM gene located on chromosome 19. Using the intensity of the expanded triplet-repeat on the chromosomes as a reference, it was estimated there were between 15-230 RNA molecules in each focus observed in DM nuclei.

Citations

Feb 22, 2005·The Journal of Biological Chemistry·Marc-André LangloisNan Sook Lee
Jul 1, 2010·Wiley Interdisciplinary Reviews. RNA·Alexa M Dickson, Carol J Wilusz
Sep 14, 2011·Artificial DNA, PNA & XNA·Takehiko ShiraishiPeter E Nielsen
May 6, 2014·Nucleic Acids Research·Olof J PetterssonChristian K Damgaard
May 7, 2015·Memórias do Instituto Oswaldo Cruz·Karen C M MoraesMaria Terezinha Bahia
Jul 7, 2011·Human Molecular Genetics·Marzena Wojciechowska, Wlodzimierz J Krzyzosiak
Jan 22, 2015·Nucleic Acids Research·Olof Joakim PetterssonChristian Kroun Damgaard
Oct 17, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M P van de CorputA K Raap
Dec 14, 2011·Nucleic Acids Research·François-Xavier LaurentJoëlle Marie
Mar 28, 2008·Experimental Cell Research·Arnaud F KleinBernard Brais
Jun 30, 2010·RNA·Kelly L RobertsonEddie L Chang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.