Jan 1, 1989

Localization of two distinct acid phosphatases in secretory ameloblasts of rat molar tooth germs

Archives of Oral Biology
S MatsuoM Akai

Abstract

Acid phosphatases were examined histochemically at the light- and electron-microscopic levels using para-nitrophenyl phosphate (pNPP) and beta-glycerophosphate (beta-GP) as substrates. By light microscopy, there was intense activity with pNPP in supranuclear and distal regions of the secretory ameloblast, and moderate or slight activity respectively in those regions with beta-GP. These enzyme activities were less at the late secretory stage of amelogenesis and disappeared at the transitional stage. By electron microscopy, acid phosphatase activity was seen in the trans side cisternae of the Golgi apparatus, in lysosome-like granules, and in small vesicles in the Tomes' processes. The activity with pNPP but not beta-GP was also localized at the plasma membrane (proximal, lateral and distal surface). Activity with beta-GP was completely inhibited by 1 mM sodium tartrate and by 1 mM NaF; activity with pNPP was inhibited by 1 mM NaF and 10 mM sodium tartrate, but not by 1 mM sodium tartrate. Thus there are at least two different acid phosphatases, one tartrate-sensitive and the other 1 mM tartrate-resistant, in the secretory ameloblast; the tartrate-resistant enzyme is plasma-membrane bound.

  • References22
  • Citations3

References

Mentioned in this Paper

Establishment and Maintenance of Localization
Acid Phosphatase
Amelogenesis
Lysosome Assembly Pathway
beta-glycerophosphoric acid, calcium salt (1: 1)
Sodium tartrate
Phosphoric Monoester Hydrolases
Golgi Apparatus
August Rats
Sodium nitrophenylphosphate

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